ZFIN ID: ZDB-FIG-070118-8
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 5 Myf5 and Myod function independently to activate progenitor lineages in muscles of the head region. Embryos co-injected with 4 ng of myf5-morpholino oligonucleotide (MO) with 4 ng of myod-MO to inhibit specifically both myf5 and myod translation, respectively, were used to observe the development of cranial muscle (A, B) and the expression of myogenin and mrf4 (D, F) at 72 hpf. Panel B was magnified from the head area of panel A. No red fluorescent protein (RFP) signal was detected in muscle primordia in the myf5 and myod double-knockdown morphants derived from the transgenic line Tg(α-actin:RFP; A, B). Similarly, the transcripts of myogenin (C vs. D) and mrf4 (E vs. F) were not detected in the myf5 and myod double-knockdown morphants (I vs. J). The myod mRNA did not rescue the formation of RFP-labeled primordia muscles in myf5 morphants (G). Similarly, the myf5 mRNA did not rescue the formation of RFP-labeled primordia muscle in myod morphants (H). For abbreviations, see the legend of Fig. 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf6 WT standard conditions Protruding-mouth cephalic musculature ISH
WT + MO1-myf5 + MO1-myod1 standard conditions Protruding-mouth Not Detected cephalic musculature ISH
myog WT standard conditions Protruding-mouth cephalic musculature ISH
WT + MO1-myf5 + MO1-myod1 standard conditions Protruding-mouth Not Detected cephalic musculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myf5 + MO1-myod1 standard conditions Protruding-mouth cephalic musculature decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.