ZFIN ID: ZDB-FIG-070118-9
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec

Fig. 6 Loss of Myod up-regulated Myf5 expression, and vice versa, in the cranial muscle that migrated from the anterior somites. The expression of myf5 was increased in sternohyoideus (sh) primordia, fin bud (fb), and posterior hypaxial muscle (phm) that migrated from the anterior somites, when embryos received myod-morpholino oligonucleotide (MO; vs. B). This consequence was consistent in trunk myogenesis. There was weak myf5 expression in the trunk of wild-type embryos at 48 hpf (C). However, myf5 appeared predominantly in the dorsal (ds) and ventral (vs) parts of somites in the myod morphants (E), indicating that myodknockdown up-regulated the expression of myf5 both in sh, which originated from trunk somite, and in trunk muscle. Similarly, the expression of myod was also enhanced in the trunk of myf5 morphants, compared to wild-type embryos (D vs. F). The myogenin expression was similar to that of myf5 in myod morphants (E, G) and to that of myod in myf5 morphants (F, H).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myf5 WT standard conditions Long-pec epaxial myotome region ISH
Long-pec hyoid muscle ISH
Long-pec hypaxial myotome region ISH
Long-pec pectoral fin musculature ISH
WT + MO1-myod1 standard conditions Long-pec epaxial myotome region ISH
Long-pec hyoid muscle ISH
Long-pec hypaxial myotome region ISH
Long-pec pectoral fin musculature ISH
myod1 WT standard conditions Long-pec somite ISH
WT + MO1-myf5 standard conditions Long-pec somite ISH
myog WT + MO1-myf5 standard conditions Long-pec epaxial myotome region ISH
Long-pec hypaxial myotome region ISH
WT + MO1-myod1 standard conditions Long-pec epaxial myotome region ISH
Long-pec hypaxial myotome region ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.