ZFIN ID: ZDB-FIG-070118-7
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-15 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-15 to Long-pec

Fig. 4 Myod is required for the development of posterior extraocular recti and ventral branchial muscles. Embryos derived from the transgenic line Tg(α-actin:RFP) (A–D) and from the wild-type strain (E–J) were used. Embryos injected with 4 ng of myod-morpholino oligonucleotide (MO) to inhibit specifically myod translation were followed to observe the development of cranial muscle (B, D) and the expression of myf5 (F, H) and myogenin (J; lateral views: A, B, E–J; ventral views: C, D) at the stages indicated. The anterior extraocular recti (io and so), dorsal branchial muscle (ah, ao, do, and lap), and sh developed normally in the myod transgenic morphant (A vs. B; C vs. D). The posterior extraocular (sr, mr, ir, and lr) and the ventral branchial muscle (ima, imp, ih, and hh) were totally lost. When wild-type embryos were injected with myod-MO, myf5 was expressed normally in the myod morphants at 30 hpf (E vs. F) and at 36 hpf (G vs. H). Whereas, the expression of myogenin was decreased in myod morphants at 58 hpf (I vs. J), indicating a reduction in myogenin-positive muscle fibers.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf5 WT standard conditions Prim-15 dorsal oblique extraocular muscle ISH
Prim-15 dorsal rectus ISH
Prim-15 lateral rectus ISH
Prim-15 pharyngeal arch ISH
Prim-15 ventral oblique extraocular muscle ISH
Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 dorsal rectus ISH
Prim-25 Not Detected lateral rectus ISH
Prim-25 pharyngeal arch ISH
Prim-25 ventral oblique extraocular muscle ISH
WT + MO1-myod1 standard conditions Prim-15 dorsal oblique extraocular muscle ISH
Prim-15 dorsal rectus ISH
Prim-15 lateral rectus ISH
Prim-15 pharyngeal arch ISH
Prim-15 ventral oblique extraocular muscle ISH
Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 dorsal rectus ISH
Prim-25 Not Detected lateral rectus ISH
Prim-25 pharyngeal arch ISH
Prim-25 ventral oblique extraocular muscle ISH
myog WT standard conditions Long-pec cephalic musculature ISH
WT + MO1-myod1 standard conditions Long-pec cephalic musculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 standard conditions Prim-15 cephalic musculature decreased amount, abnormal
Prim-25 cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.