ZFIN ID: ZDB-FIG-070118-11
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 8 Myf5 is required for the migration of the sternohyoideus (sh) primordia from anterior somites. The transcripts of myf5 (A), myod (B), and met (C) were detected in sternohyoideus (sh) premordia, fin bud (fb), and posterior hypaxial muscle (phm), that were derived from anterior somites at 36 hpf. Embryos injected with myf5-morpholino oligonucleotide (MO) and myod-MO to inhibit specifically myf5 and myod translations, respectively, expressed met at the stages indicated. The expression of met was almost lost in myf5 morphants; only slight signals were detected in the phm (D; arrow). However, met was expressed normally in myod morphants (E). At 48 hpf, myogenin was expressed in the sh, fb, and phm of wild-type (F) and myod morphants (H), but myogenin was not expressed in the sh, fb, or phm of myf5 morphants (G). Instead, the expression of myogenin was up-regulated in the trunk of myf5 morphants. Embryos derived from the transgenic line Tg(α-actin:RFP; I–K) were used. At 72 hpf, the sh, fb, and phm showing red fluorescent signal were clearly observed both in wild-type (I; arrow) and myod morphants (K; arrow) but not in myf5 morphants (J). For abbreviations, see the legend to Fig. 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
met WT standard conditions Prim-25 hyoid muscle ISH
Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
WT + MO1-myf5 standard conditions Prim-25 Not Detected hyoid muscle ISH
Prim-25 hypaxialis ISH
Prim-25 Not Detected pectoral fin musculature ISH
WT + MO1-myod1 standard conditions Prim-25 hyoid muscle ISH
Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
myf5 WT standard conditions Prim-25 hyoid muscle ISH
Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
myod1 WT standard conditions Prim-25 hyoid muscle ISH
Prim-25 hypaxialis ISH
Prim-25 pectoral fin musculature ISH
myog WT standard conditions Long-pec hyoid muscle ISH
Long-pec hypaxialis ISH
Long-pec pectoral fin musculature ISH
WT + MO1-myf5 standard conditions Long-pec Not Detected hyoid muscle ISH
Long-pec Not Detected hypaxialis ISH
Long-pec Not Detected pectoral fin musculature ISH
Long-pec trunk ISH
WT + MO1-myod1 standard conditions Long-pec hyoid muscle ISH
Long-pec hypaxialis ISH
Long-pec pectoral fin musculature ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myf5 standard conditions Prim-25 cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
WT + MO1-myod1 standard conditions Prim-25 cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.