ZFIN ID: ZDB-FIG-070118-5
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec

Fig. 2 The temporal expressions of myf5 and myod during cranial muscle development. The temporal expressions of myf5 (A–C, E, G, I) and myod (D, F, H, J) transcripts were analyzed by whole-mount in situ hybridization in zebrafish embryos. The transcript of myf5 was detected in the craniofacial region at 24 hpf (A, arrow); in the io, the precursor of lr, and 1st arch (lr/1st arch) at 30 hpf (B); in the so, io, lr, 1st, 2nd, and 3rd arches at 32 hpf (C); in the so, io, sh, 2nd, and 3rd arches at 36 hpf (E); and in the so, io, and sh at 42–48 hpf (G, I). myf5 was not expressed in the lr and 1st arch at 36 hpf (E). Meanwhile, myod was expressed in the craniofacial muscles mr/ir/sr, lr, and 1st arch at 32 hpf (D); in the mr/ir, sr, lr, MP, IM, CHD, and CHV at 36 hpf (F); and in all the cranial muscle at 42–48 hpf (H, J). CD: the constrictor dorsalis, which differentiates to lap and do; CHD: the constrictor hyoideus dorsalis, which differentiates to ah and ao; CHV: the constrictor hyoideus ventralis, which differentiates to ih and hh; IM: the intermandibularis, which differentiates to ima and imp; MP: the masticatory plate, which differentiates to CD and am. lr, lateral rectus; for other abbreviations, see the legend of Fig. 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf5 WT standard conditions Prim-5 lateral rectus ISH
Prim-5 pharyngeal arch ISH
Prim-5 ventral oblique extraocular muscle ISH
Prim-15 dorsal oblique extraocular muscle ISH
Prim-15 hyoid muscle ISH
Prim-15 lateral rectus ISH
Prim-15 pharyngeal arch ISH
Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 hyoid muscle ISH
Prim-25 Not Detected lateral rectus ISH
Prim-25 pharyngeal arch ISH
Prim-25 ventral oblique extraocular muscle ISH
High-pec dorsal oblique extraocular muscle ISH
High-pec hyoid muscle ISH
High-pec ventral oblique extraocular muscle ISH
WT + MO1-myod1 standard conditions Long-pec cephalic musculature ISH
Long-pec constrictor dorsalis ISH
myod1 WT standard conditions Prim-15 dorsal rectus ISH
Prim-15 lateral rectus ISH
Prim-15 medial rectus ISH
Prim-15 pharyngeal arch ISH
Prim-15 ventral rectus ISH
Prim-25 dorsal rectus ISH
Prim-25 hyoid muscle ISH
Prim-25 intermandibularis ISH
Prim-25 lateral rectus ISH
Prim-25 mandibular muscle ISH
Prim-25 medial rectus ISH
Prim-25 ventral rectus ISH
High-pec cephalic musculature ISH
Long-pec cephalic musculature ISH
Long-pec constrictor dorsalis ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.