ZFIN ID: ZDB-FIG-070118-6
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 3 Myf5 is required for cranial muscle development, except the primordia of mr/ir/sr. Embryos derived from the transgenic line Tg(α-actin:RFP; A, B) and from the wild-type strain (C–J) were used. All the embryos were lateral views except panels E and F, which were dorsal–lateral view. The embryos injected with 4 ng of myf5-morpholino oligonucleotide (MO) to inhibit myf5 translation specifically, were studied to observe the development of cranial muscle (B) and the expression of myod, et1, and myogenin (D, F, H, J) at the stages indicated. Red fluorescent protein (RFP) signal was detected only in the mr/ir and sr primordia in the myf5 transgenic morphant (A vs. B). myod was expressed only in the mr/ir/sr primordia in the myf5 wild-type morphant at 36 hpf (C vs. D) and at 58 hpf (G vs. H). Similarly, myogenin was detected only in the mr/ir/sr primordia in myf5 morphant (I vs. J). However, the et1 transcript was changed little in the ventral arch mesoderm core in the myf5 morphant (E vs. F, arrows), indicating that the development of the ventral arch mesoderm core in myf5 morphant was normal.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
edn1 WT standard conditions Prim-25 pharyngeal arch ISH
WT + MO1-myf5 standard conditions Prim-25 pharyngeal arch ISH
myod1 WT standard conditions Prim-25 adductor hyohyoid ISH
Prim-25 dorsal rectus ISH
Prim-25 hyoid muscle ISH
Prim-25 lateral rectus ISH
Prim-25 mandibular muscle ISH
Prim-25 medial rectus ISH
Prim-25 pharyngeal musculature ISH
Prim-25 ventral rectus ISH
Long-pec cephalic musculature ISH
WT + MO1-myf5 standard conditions Prim-25 dorsal rectus ISH
Prim-25 medial rectus ISH
Prim-25 ventral rectus ISH
Long-pec dorsal rectus ISH
Long-pec medial rectus ISH
Long-pec ventral rectus ISH
myog WT standard conditions Long-pec cephalic musculature ISH
WT + MO1-myf5 standard conditions Long-pec dorsal rectus ISH
Long-pec medial rectus ISH
Long-pec ventral rectus ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myf5 standard conditions Prim-25 cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.