ZFIN ID: ZDB-FIG-070118-10
Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish. Developmental Biology   299(2):594-608 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Long-pec

Fig. 7 Myf5 is required for the forward migration of the superior oblique (so) and inferior oblique (io) primordia toward the anterior eye region. Embryos derived from the transgenic line Tg(myf5:EGFP), in which an upstream region of zebrafish myf5 was fused with enhanced green fluorescent protein (EGFP), were used to trace the migration of cranial muscle that express myf5. The primordia of io and so labeled with EGFP were clearly visible in the embryos derived from the transgenic line Tg(myf5:EGFP) at 36–48 hpf under fluorescent microscopy (A, E, I; arrows). Whole-mount in situ hybridization of wild-type zebrafish embryos showed that the expression of met, a cell marker of migration, was positive in the io and so at 36–48 hpf (B, F, J; arrows). Embryos injected with myf5-morpholino oligonucleotide (MO) and myod-MO to inhibit myf5 and myod translations, respectively, expressed met at the stages indicated. The met transcript was not expressed in the io and so of myf5 morphants (C, G, K), but met was expressed normally in the io and so of myod morphants (D, H, L; arrows). For abbreviations, see the legend of Fig. 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
met WT standard conditions Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 ventral oblique extraocular muscle ISH
High-pec dorsal oblique extraocular muscle ISH
High-pec ventral oblique extraocular muscle ISH
Long-pec dorsal oblique extraocular muscle ISH
Long-pec ventral oblique extraocular muscle ISH
WT + MO1-myf5 standard conditions Prim-25 Not Detected dorsal oblique extraocular muscle ISH
Prim-25 Not Detected ventral oblique extraocular muscle ISH
High-pec Not Detected dorsal oblique extraocular muscle ISH
High-pec Not Detected ventral oblique extraocular muscle ISH
Long-pec Not Detected dorsal oblique extraocular muscle ISH
Long-pec Not Detected ventral oblique extraocular muscle ISH
WT + MO1-myod1 standard conditions Prim-25 dorsal oblique extraocular muscle ISH
Prim-25 ventral oblique extraocular muscle ISH
High-pec dorsal oblique extraocular muscle ISH
High-pec ventral oblique extraocular muscle ISH
Long-pec dorsal oblique extraocular muscle ISH
Long-pec ventral oblique extraocular muscle ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myf5 standard conditions Prim-25 cephalic musculature decreased amount, abnormal
High-pec cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
WT + MO1-myod1 standard conditions Prim-25 cephalic musculature decreased amount, abnormal
High-pec cephalic musculature decreased amount, abnormal
Long-pec cephalic musculature decreased amount, abnormal
Acknowledgments:
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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.