FIGURE

Fig. 7

ID

ZDB-FIG-241126-45

Publication

Schmitner et al., 2024 - Conserved glucokinase regulation in zebrafish confirms therapeutic utility for pharmacologic modulation in diabetes

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Fig. 7

Activation of Gck ameliorates hyperglycemia.

a Experimental setup and timeline created in https://BioRender.com. b Glucose measurements of pdx1^-/- after 5 days treatment with either 2 µM Dorzagliatin or 500 µM D-Mannoheptulose reveal a glucose lowering effect of Dorzagliatin (N = 4) as shown in a dot plot with indicated mean. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. c Measurement of Gck activity in pdx1^-/- following treatments as indicated (N > 3) as shown in a dot plot with indicated mean. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. dgck:GFP expression following Dorzagliatin or D-Mannoheptulose treatment in pdx1^-/- larvae. Stress-induced fluorescence signal quantification (e) and representative images (f) showing gck:GFP and oxidative stress as assessed by EpRE:RFP expression in the liver of control DMSO-treated and pdx1^-/- treated with DMSO, Dorzagliatin or D-Mannoheptulose. (Liver is outlined in white). Dot and bar plot showing mean with SD. Statistical significance assessed by One-way ANOVA. P-values of multiple comparisons are indicated. Scale bar: 100 µm.

Expression Data

Gene:	GFP
Fish:	ml203Tg; ml204Tg pdx1^sa280/sa280; ml203Tg; ml204Tg
Conditions:	chemical treatment by environment: enzyme activator chemical treatment by environment: D-manno-heptulose
Anatomical Term:	liver
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pdx1^sa280/sa280 ml203Tg; ml204Tg pdx1^sa280/sa280; ml203Tg; ml204Tg
Conditions:	chemical treatment by environment: enzyme activator chemical treatment by environment: D-manno-heptulose
Observed In:	liver liver reactive oxygen species whole organism glucose
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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