ZFIN ID: ZDB-FIG-210216-64
Lou et al., 2020 - Elevated 4-hydroxynonenal induces hyperglycaemia via Aldh3a1 loss in zebrafish and associates with diabetes progression in humans. Redox Biology   37:101723 Full text @ Redox Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 5 Fig. 5. Primary pancreas was disrupted by ALDH inhibition, aldh3a1 transient silencing and permanent knockout in zebrafish larvae. (A). ALDH inhibition by DEAB treatment, and endogenous aldh3a1 expression silencing induced decreased dimensions of the primary pancreas in Tg(hb9:GFP) and a reduced sign of β cell mass area in Tg(ins:nfsB-mCherry) zebrafish larvae at 72 hpf. White box indicates the primary pancreas; Grey box indicates the β cell mass area; White scale bar = 100 μm, grey scale bar = 50 μm. (B–C). Quantification of area size of the primary pancreas (B) and β cell mass(C) in violin plots, n = 12–33 per group. (D–E). Similar to pdx1 morpholino mediated silencing (E), ins mRNA expression was decreased significantly in both aldh3a1 silencing(D) and Aldh3a1 permanent knockout (E) zebrafish larvae at 48 hpf, while insb was unaltered. Expression of mRNA was analysed by RT-qPCR and was normalized to b2m. The average values of Control-Mo injected (D) and aldh3a1+/+ zebrafish larvae (E) were standardized to 1, n = 3–6 clutches with 30 larvae per group. 6 ng of each morpholino was injected into the one-cell stage of zebrafish embryos, respectively. For statistical analysis one-way ANOVA followed by Sidak's multiple comparison test was applied, *p < 0.05, ***p < 0.001, ****p < 0.0001. Mo, morpholino; NS, not significant; ins, preproinsulin; insb, preproinsulin b; DEAB, N,N-diethylaminobenzaldehyde.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins WT control Long-pec whole organism RTPCR
WT + MO1-aldh3a1 standard conditions Long-pec whole organism RTPCR
WT + MO1-pdx1 standard conditions Long-pec whole organism RTPCR
WT + MO2-aldh3a1 standard conditions Long-pec whole organism RTPCR
aldh3a1zf3260/zf3260 standard conditions Long-pec whole organism RTPCR
insb WT control Long-pec whole organism RTPCR
WT + MO1-aldh3a1 standard conditions Long-pec whole organism RTPCR
WT + MO1-pdx1 standard conditions Long-pec whole organism RTPCR
WT + MO2-aldh3a1 standard conditions Long-pec whole organism RTPCR
aldh3a1zf3260/zf3260 standard conditions Long-pec whole organism RTPCR
pdx1 WT control Long-pec whole organism RTPCR
WT + MO1-aldh3a1 standard conditions Long-pec whole organism RTPCR
WT + MO1-pdx1 standard conditions Long-pec whole organism RTPCR
WT + MO2-aldh3a1 standard conditions Long-pec whole organism RTPCR
aldh3a1zf3260/zf3260 standard conditions Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-aldh3a1 standard conditions Long-pec whole organism ins expression decreased amount, abnormal
Long-pec whole organism pdx1 expression decreased amount, abnormal
WT + MO1-pdx1 standard conditions Long-pec whole organism ins expression decreased amount, abnormal
WT + MO2-aldh3a1 standard conditions Long-pec whole organism ins expression decreased amount, abnormal
Long-pec whole organism pdx1 expression decreased amount, abnormal
aldh3a1zf3260/zf3260 standard conditions Long-pec whole organism ins expression decreased amount, abnormal
Long-pec whole organism pdx1 expression decreased amount, abnormal
jh4Tg chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde Protruding-mouth pancreatic B cell decreased amount, abnormal
jh4Tg + MO1-pdx1 standard conditions Protruding-mouth pancreatic B cell decreased amount, abnormal
jh4Tg + MO2-aldh3a1 standard conditions Protruding-mouth pancreatic B cell decreased amount, abnormal
ml2Tg chemical treatment by environment: 3-allyl-2-[2-(diethylamino)ethoxy]benzaldehyde Protruding-mouth pancreas decreased size, abnormal
ml2Tg + MO1-aldh3a1 standard conditions Protruding-mouth pancreas decreased size, abnormal
ml2Tg + MO1-pdx1 standard conditions Protruding-mouth pancreas decreased size, abnormal
ml2Tg + MO2-aldh3a1 standard conditions Protruding-mouth pancreas decreased size, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.