ZFIN ID: ZDB-FIG-210216-67
Lou et al., 2020 - Elevated 4-hydroxynonenal induces hyperglycaemia via Aldh3a1 loss in zebrafish and associates with diabetes progression in humans. Redox Biology   37:101723 Full text @ Redox Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 8 Fig. 8. Defective 4-HNE detoxification and the elevation of 4-HNE induced the imbalance of glucose homeostasis after Aldh3a1 loss. (A). Aldh3a1−/− zebrafish larvae showed unaltered ALDH activity, Glo1 activity when MG as substrate and unchanged MG amount at 96 hpf. (B–C). Aldh3a1−/− zebrafish larvae showed decreased ALDH activity when 4-HNE as substrate, increased 4-HNE amount and decreased free cysteine at 96 hpf, but pdx1 morphants showed no significant change in ALDH activity and 4-HNE amount. (D–F). 10 μM 4-HNE treatment in wild type zebrafish larvae caused: (D). Decreased ins and pdx1 mRNA expression at 48 hpf. Expression of mRNA was analysed by RT-qPCR and was normalized to b2m; (E). Reduced glycogenic amino acids Asn and Met at 96hpf; (F). Elevated glucose at 120 hpf. (G). 4-HNE and ALDH dependent 4-HNE detoxification ability were not changed in zebrafish larvae with pdx1 silencing at 96hpf. (H). Concise mechanism flow chart showed the consequence of defective 4-HNE detoxification after Aldh3a1 loss. n = 3–7 clutches as each plot showed with 30–50 larvae per group. For statistical analysis paired samples t-tests were applied. *p < 0.05, **p < 0.01, ***p < 0.0010.4-HNE, 4-Hydroxynonenal; Glo1, glyoxalase 1; MG, methylglyoxal.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ins WT control Long-pec whole organism RTPCR
WT chemical treatment by environment: 4-hydroxynon-2-enal Long-pec whole organism RTPCR
insb WT control Long-pec whole organism RTPCR
WT chemical treatment by environment: 4-hydroxynon-2-enal Long-pec whole organism RTPCR
pdx1 WT control Long-pec whole organism RTPCR
WT chemical treatment by environment: 4-hydroxynon-2-enal Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment by environment: 4-hydroxynon-2-enal Long-pec whole organism ins expression decreased amount, abnormal
Long-pec whole organism pdx1 expression decreased amount, abnormal
Day 5 whole organism glucose increased amount, abnormal
aldh3a1zf3260/zf3260 standard conditions Day 4 whole organism 4-hydroxynon-2-enal increased amount, abnormal
Day 4 whole organism asparagine decreased amount, abnormal
Day 4 whole organism cysteine decreased amount, abnormal
Day 4 whole organism methionine decreased amount, abnormal
Day 4 whole organism methylglyoxal amount, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Redox Biol.