ZFIN ID: ZDB-FIG-210216-68
Lou et al., 2020 - Elevated 4-hydroxynonenal induces hyperglycaemia via Aldh3a1 loss in zebrafish and associates with diabetes progression in humans. Redox Biology   37:101723 Full text @ Redox Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Protruding-mouth to Day 5

Fig. 9 Fig. 9. Exogenous 4-HNE disrupted the pancreas, induced hyaloid vascular alterations, and can be rescued via l-Carnosine and PK11195 treatment, respectively. (A). 10 μM 4-HNE treatment induced decreased dimensions of the primary pancreas in Tg(hb9:GFP) and reduced trend of β cell mass in Tg(ins:nfsB-mCherry) zebrafish larvae at 72 hpf, which were rescued by 5 mM Carnosine but not 10 μM PK11195 intervention. Grey box indicates the β cell mass area; White scale bar = 100 μm, grey scale bar = 50 μm. (B–C). Quantification of area size of the early pancreas (B) and β cell mass (C) in violin plots, n = 17–26 per group. (D) 4-HNE incubation led to increased IOC branch diameters (white arrow) and sprouts (white delta) in hyaloid vasculature of Tg(fli1:EGFP) zebrafish larvae at 120 hpf, which were rescued via 10 μM PK11195 intervention. White scale bar = 20 μm. (E–F). Quantification of the IOC branch diameters and sprouts in violin plots, n = 10–20 per group. For statistical analysis one-way ANOVA followed by Sidak's multiple comparison test was applied, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
jh4Tg chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreatic B cell decreased amount, abnormal
jh4Tg chemical treatment by environment: carnosine, chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreatic B cell amount, ameliorated
jh4Tg chemical treatment by environment: PK-11195, chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreatic B cell decreased amount, abnormal
ml2Tg chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreas decreased size, abnormal
ml2Tg chemical treatment by environment: carnosine, chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreas size, ameliorated
ml2Tg chemical treatment by environment: PK-11195, chemical treatment by environment: 4-hydroxynon-2-enal Protruding-mouth pancreas decreased size, abnormal
y1Tg chemical treatment by environment: 4-hydroxynon-2-enal Day 5 ocular blood vessel increased diameter, abnormal
Day 5 ocular blood vessel sprouting angiogenesis increased occurrence, abnormal
y1Tg chemical treatment by environment: PK-11195, chemical treatment by environment: 4-hydroxynon-2-enal Day 5 ocular blood vessel diameter, ameliorated
Day 5 ocular blood vessel sprouting angiogenesis occurrence, ameliorated
Acknowledgments:
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