ZFIN ID: ZDB-FIG-171114-20
Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis. Scientific Reports   6:25300 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 4

Knockdown of syn4 expression promotes the generation of neurons.

(AR'') WISH results of elavl3, islet1, and islet2a at 24 hpf and 48 hpf in zebrafish. (EE'') The expression of elavl3, (KK'') islet1, (QQ'') islet2a in spinal cord were increased in syn4 morphants at 48 hpf. co-injecting syn4 mRNA was able to rescue the defects. (S) sqRT-PCR analyzes of the transcript level of elavl3, islet1 and islet2a. (T–V) Densitometric quantification of sqRT-PCR. The syn4 morphants shown a higher expression level of elavl3, islet1 and islet2a (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). ALLG: anterior lateral line ganglion, Hb: hindbrain, Mb: midbrain, sc: spinal cord. Lateral views, dorsal to the top and anterior to the left in (A–F',G–L',M–R'). Dorsal view, anterior to the left in (D''–F'',J''–L'',P''–R'').

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
elavl3 AB control Prim-5 neuron ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Long-pec hindbrain ISH
Long-pec midbrain ISH
Long-pec neuron ISH
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 neuron ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Long-pec midbrain ISH
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
isl1a AB control Prim-5 MiP motor neuron ISH
Prim-5 RoP motor neuron ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Long-pec anterior lateral line ganglion ISH
Long-pec MiP motor neuron ISH
Long-pec RoP motor neuron ISH
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 MiP motor neuron ISH
Prim-5 RoP motor neuron ISH
Prim-5 spinal cord ISH
Prim-5 whole organism RTPCR
Long-pec anterior lateral line ganglion ISH
Long-pec spinal cord ventral region ISH
Long-pec whole organism RTPCR
isl2a AB control Prim-5 CaP motoneuron ISH
Prim-5 spinal cord ISH
Prim-5 VaP motor neuron ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 CaP motoneuron ISH
Prim-5 spinal cord ISH
Prim-5 VaP motor neuron ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord ventral region ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sdc4 standard conditions Long-pec midbrain elavl3 expression increased amount, abnormal
Long-pec spinal cord elavl3 expression increased amount, abnormal
Long-pec spinal cord ventral region isl1a expression increased amount, abnormal
Long-pec spinal cord ventral region isl2a expression increased amount, abnormal
Long-pec whole organism elavl3 expression increased amount, abnormal
Long-pec whole organism isl1a expression increased amount, abnormal
Long-pec whole organism isl2a expression increased amount, abnormal
Acknowledgments:
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