ZFIN ID: ZDB-FIG-171114-17
Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis. Scientific Reports   6:25300 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Protruding-mouth

Fig. 1

Syn4 expression pattern analysis in zebrafish.

(A) sqRT-PCR reveals that syn4 is expressed throughout embryonic developmental stages. (B–M) WISH shows the spatiotemporal expression pattern of syn4. (B–D) Syn4 is ubiquitously distributed before the gastrulation. (E) In 75% epiboly, syn4 is expressed in the thin evacuation zone and prechordal plate. (F–K) Syn4 is expressed in ventral diencephalon, midbrain, hind brain and neural crest during Segmentation Period. (L,M) In 48 hpf, syn4 is restricted to the ventricular zone in the forebrain, posterior midbrain and hindbrain. (N) Negative control of syn4 at 48 hpf. EZ: thin evacuation zone, FV: ventricular zone in the forebrain, Hb: hindbrain, Mb: midbrain, MHB: mid-hindbrain boundary, pp: prechordal plate, PM: posterior midbrain, R: rhombomeres, som: somites, sc: spinal cord, vd: ventral diencephalon. Lateral view, dorsal to the right in (B–E), dorsal view, anterior to the left in (G,I,K,M) lateral view, dorsal to the right and anterior to the top in (F), lateral view, dorsal to the top and anterior to the left in (H,J,M).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gapdh AB standard conditions 1-cell whole organism RTPCR
1k-cell whole organism RTPCR
75%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
sdc4 AB standard conditions 1-cell whole organism ISH
1-cell whole organism RTPCR
1k-cell whole organism RTPCR
30%-epiboly whole organism ISH
Shield whole organism ISH
75%-epiboly prechordal plate ISH
75%-epiboly whole organism RTPCR
10-13 somites whole organism RTPCR
Prim-5 diencephalon ventral region ISH
Prim-5 hindbrain ISH
Prim-5 midbrain ISH
Prim-5 neural crest ISH
Prim-5 whole organism RTPCR
Long-pec forebrain ventricular zone ISH
Long-pec hindbrain ventricular zone ISH
Long-pec midbrain ventricular zone ISH
Long-pec whole organism RTPCR
Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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