ZFIN ID: ZDB-FIG-171114-19
Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis. Scientific Reports   6:25300 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 3

Loss of syn4 increased neuroglial cells.

(A–R'') WISH results of gfap (A–F''), slc1a3a (GL'') and olig2 (M–R'') at 24 hpf and 48 hpf in zebrafish. (DF') The expression of gfap, (JL'') slc1a3a, (QR'') olig2 are higher in the syn4 morphants than controls at 48 hpf. (S) sqRT-PCR analyzes of the transcript level of gfap, slc1a3a and olig2. (TW) Densitometric quantification of sqRT-PCR. The syn4 morphants shown a higher expression level of gfap, slc1a3a and olig2 (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). FV: ventricular zone in forebrain, Hb: hindbrain, PM: posterior midbrain, sc: spinal cord. Lateral views, dorsal to the top and anterior to the left in (A–C,D–F,G–I,J–L',M–O,P–R,P''–R''), Dorsal view, anterior to the left in (D'–F',G'–I',J''–L'',P'–R').

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gfap AB control Prim-5 astrocyte ISH
Prim-5 whole organism RTPCR
Long-pec forebrain ventricular zone ISH
Long-pec midbrain ventricular zone ISH
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 astrocyte ISH
Prim-5 whole organism RTPCR
Long-pec forebrain ventricular zone ISH
Long-pec midbrain ventricular zone ISH
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
olig2 AB control Prim-5 oligodendrocyte ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 oligodendrocyte ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord ISH
Long-pec whole organism RTPCR
slc1a3a AB control Prim-5 radial glial cell ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord dorsal region ISH
Long-pec whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 radial glial cell ISH
Prim-5 whole organism RTPCR
Long-pec spinal cord dorsal region ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sdc4 standard conditions Long-pec forebrain ventricular zone gfap expression increased amount, abnormal
Long-pec midbrain ventricular zone gfap expression increased amount, abnormal
Long-pec spinal cord gfap expression increased amount, abnormal
Long-pec spinal cord olig2 expression increased amount, abnormal
Long-pec spinal cord dorsal region slc1a3a expression increased amount, abnormal
Long-pec whole organism gfap expression increased amount, abnormal
Long-pec whole organism olig2 expression increased amount, abnormal
Long-pec whole organism slc1a3a expression increased amount, abnormal
Acknowledgments:
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