ZFIN ID: ZDB-FIG-171114-18
Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis. Scientific Reports   6:25300 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 2

Knockdown of syn4 expression up-regulated the expression of nestin.

(A–C) Expression of nestin at 24 hpf, lateral view. (D–F) Expression of nestin at 48 hpf, lateral view. (EE”) Nestin transcript level was increased significantly in syn4 morphants, dorsal view. (FF”) The defective phenotypes were rescued by co-injecting syn4 mRNA. (G) sqRT-PCR showed the similar results. (H) Densitometric quantification of sqRT-PCR. The relative nestin expressing level is significantly higher in syn4 morphants (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student’s unpaired t-test). ALLG: anterior lateral line ganglion, FV: ventricular zone in forebrain, Hb: hindbrain, OG: octaval ganglion, sc: spinal cord, VMP: ventral motoneuron precursors.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nes AB control Prim-5 neuronal stem cell ISH
Prim-5 whole organism RTPCR
Long-pec forebrain ventricular zone ISH
Long-pec hindbrain ventricular zone ISH
Long-pec neuronal stem cell ISH
Long-pec Not Detected whole organism RTPCR
AB + MO3-sdc4 standard conditions Prim-5 neuronal stem cell ISH
Prim-5 whole organism RTPCR
Long-pec forebrain ventricular zone ISH
Long-pec hindbrain ventricular zone ISH
Long-pec neuronal stem cell ISH
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sdc4 standard conditions Long-pec forebrain ventricular zone nes expression increased amount, abnormal
Long-pec hindbrain ventricular zone nes expression increased amount, abnormal
Long-pec neuronal stem cell nes expression increased amount, abnormal
Long-pec whole organism nes expression increased amount, abnormal
Acknowledgments:
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