Whole-mount immunohistochemistry staining of PCNA and H3S10 in zebrafish embryos.
(A–D'') Immunostaining of PCNA and H3S10 in 24 hpf and 48 hpf embryos, the box regions are magnified. (E,F) Quantification of PCNA-positive cells (E) and H3S10 -positive cells (F) in different treatment groups. Both PCNA positive cells and H3S10 positive cells are considerably increased in syn4 morphants and the defects were partially rescued by co-injection with syn4 mRNA. For each group, 18 embryos are scored. (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). (G) The double WISH with H3S10 and olig2. Arrows label the H3S10 positive cells, the box regions are magnified. Lateral views, dorsal to the top and anterior to the left in (A–D'',G).