ZFIN ID: ZDB-FIG-171114-21
Luo et al., 2016 - Syndecan-4 modulates the proliferation of neural cells and the formation of CaP axons during zebrafish embryonic neurogenesis. Scientific Reports   6:25300 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5

Whole-mount immunohistochemistry staining of PCNA and H3S10 in zebrafish embryos.

(AD'') Immunostaining of PCNA and H3S10 in 24 hpf and 48 hpf embryos, the box regions are magnified. (E,F) Quantification of PCNA-positive cells (E) and H3S10 -positive cells (F) in different treatment groups. Both PCNA positive cells and H3S10 positive cells are considerably increased in syn4 morphants and the defects were partially rescued by co-injection with syn4 mRNA. For each group, 18 embryos are scored. (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). (G) The double WISH with H3S10 and olig2. Arrows label the H3S10 positive cells, the box regions are magnified. Lateral views, dorsal to the top and anterior to the left in (AD'',G).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
olig2 AB control Long-pec spinal cord ISH
AB + MO3-sdc4 standard conditions Long-pec spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sdc4 standard conditions Long-pec spinal cord olig2 expression increased amount, abnormal
Acknowledgments:
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