ZFIN ID: ZDB-IMAGE-171114-4
Figures for Luo et al., 2016

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Fig. 4

Knockdown of syn4 expression promotes the generation of neurons.

(AR'') WISH results of elavl3, islet1, and islet2a at 24 hpf and 48 hpf in zebrafish. (EE'') The expression of elavl3, (KK'') islet1, (QQ'') islet2a in spinal cord were increased in syn4 morphants at 48 hpf. co-injecting syn4 mRNA was able to rescue the defects. (S) sqRT-PCR analyzes of the transcript level of elavl3, islet1 and islet2a. (T–V) Densitometric quantification of sqRT-PCR. The syn4 morphants shown a higher expression level of elavl3, islet1 and islet2a (mean ± s.e.m, n = 3, ***P < 0.001, **P < 0.01, *P < 0.05, ns = not significant, Student's unpaired t-test). ALLG: anterior lateral line ganglion, Hb: hindbrain, Mb: midbrain, sc: spinal cord. Lateral views, dorsal to the top and anterior to the left in (A–F',G–L',M–R'). Dorsal view, anterior to the left in (D''–F'',J''–L'',P''–R'').

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