Serpine1 is upregulated in the injury endocardium and its inhibition does not interfere with blood clot or collagen deposition. (A, B) ISH against serpine1, showing no expression in the uninjured heart, moderate levels at 7 dpci but almost no expression at 14 dpci. (C) FISH against serpine1 combined with IHC against GFP and MF20 on sections of ET33-mi60a transgenic hearts at 24 hpci. Serpine1 is strongly expressed in GFP+ endocardial cells adjacent and within the injury site but not in remote regions at 24 hpci. The boxed area is magnified on the right hand panel. (D) qPCR analysis showing higher serpine1 mRNA levels in injured ventricles at 7 dpci in RO-treated fish than in DMSO-treated fish (mean± s.d., t-test, *P<0.05). (E) AFOG stained TP- or DMSO treated hearts indicating similar fibrin deposition at the injury site (is) in both groups at 7dpci. (F) IHC for collagen 1 (Col1) and Mef2 showing similar amount of collagen at the injury site upon TP or DMSO-treatment. (G) Scatter plot showing the area covered by collagen relative to the injury site area in hearts treated with TP or DMSO. (dotted line = mean± s.d, t-test, not significant). (H) ISH in hearts treated with TP or DMSO (regime is indicated on top) showing similar expression of aldh1a2 in the injury endocardium. Dotted lines delineate the injury site (is). Scale bar: 100 µm; 20 µm in the amplified view in C.
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