FIGURE

Fig. 6

ID
ZDB-FIG-170516-26
Publication
Münch et al., 2017 - Notch signalling restricts inflammation and serpine1 expression in the dynamic endocardium of the regenerating zebrafish heart.
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Fig. 6

Notch signalling regulates cardiomyocyte proliferation. (A) ET33-mi60a;myl7:mRFP ventricle, volume rendering and optical section of a region of the inner injury border. Endocardial cells surround injury-adjacent cardiomyocytes (arrows) and precede into the wound. (B) IHC showing endocardial cells (GFP+) in contact with myocardial protrusions (tropomyosin+; blue arrowheads). (C,D) IHC against BrdU and Mef2 (C). Heat shock regime is indicated at the top. Scatter plot (D) of percentage of BrdU+ wound-adjacent cardiomyocytes (Mef2+; arrowheads between dotted lines). Mean±s.d.; t-test,**P<0.01. (E,F) Mef2 IHC showing a higher density of wound-adjacent cardiomyocytes (between the dotted lines) in Tg(UAS:NICD) than in control hearts (E), as quantified in F. Mean±s.d.; t-test, *P<0.05. (G) ISH showing higher numbers of nkx2.5+ wound-adjacent cardiomyocytes (between dotted lines) in Tg(UAS:NICD) hearts. (H) RNA-seq data showing differential myocardial gene expression in RO-treated hearts at 3 dpci. (I) ISH showing mycb expression in injury-adjacent cardiomyocytes (arrowheads) in DMSO-treated but not in RO-treated hearts (asterisks). (J) qPCR levels of fosab in the injured heart. Mean±s.d.; t-test, *P<0.05. LOF, loss of function. (K) MF20 IHC and mylk3 ISH on heart sections. RO treatment decreased the numbers of mylk3 cardiomyocytes adjacent to the injury site (arrowheads). (L) Scatter plot showing the percentage of mylk3 wound-adjacent (50 µm) cardiomyocytes. Mean±s.d.; t-test, **P<0.01. (M) qPCR analysis of mylk3 and tcap levels in the injured heart. Mean±s.d.; t-test, ***P<0.001. GOF, gain of function. Dotted lines delineate the injury site (is). Boxed areas are magnified in insets. Scale bars: 100 µm, except 25 µm in magnified views.

Expression Data
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage: Adult

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Conditions:
Observed In:
Stage: Adult

Phenotype Detail
Acknowledgments
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