ZFIN ID: ZDB-FIG-110812-42
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. S1

netrin 1a and dcc morphants do not form a complete thoracic duct. (A,B,D,E) 5 dpf fli1a:egfpy1 larvae; reversed contrast, confocal projections. (A2,B2,D2,E2) Enlargements of the boxed regions from A,B,D,E. (A-A2,D-D2) The thoracic duct (TD) forms normally (arrows) between the dorsal aorta (DA) and posterior cardinal vein (PCV) in uninjected control embryos. (B,B2,E,E2) The TD is absent in netrin1aSBMO or dccTBMO morphants (open arrowheads). (C,F) Quantitation of partial or complete TD formation in control embryos (77%), netrin1aSBMO morphants (25%) and dccTBMO morphants (45±19%; mean±s.e.m.); P-values, Mann-Whitney U test. n, number of hemisegments (number of embryos). PAC, parachordal chain; DA, dorsal aorta; PCV, posterior cardinal vein. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg/+ standard conditions Day 5 dorsal aorta IFL
Day 5 parachordal vessel IFL
Day 5 posterior cardinal vein IFL
Day 5 thoracic duct IFL
y1Tg/+ + MO1-dcc standard conditions Day 5 dorsal aorta IFL
Day 5 parachordal vessel IFL
Day 5 posterior cardinal vein IFL
y1Tg/+ + MO2-ntn1a standard conditions Day 5 dorsal aorta IFL
Day 5 parachordal vessel IFL
Day 5 posterior cardinal vein IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg/+ + MO1-dcc standard conditions Day 5 lymph vessel development process quality, abnormal
Day 5 thoracic duct aplastic, abnormal
y1Tg/+ + MO2-ntn1a standard conditions Day 5 lymph vessel development process quality, abnormal
Day 5 thoracic duct aplastic, abnormal
Acknowledgments:
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