ZFIN ID: ZDB-FIG-110812-41
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 7

Preventing differentiation or laser ablation of motoneurons prevents PAC formation. (A-F) Lateral views of 48 hpf fli1a:egfpy1 zebrafish embryos either uninjected or injected with olig2 splice-blocking MO (olig2SBMO) showing labeled vessels (green) and neurons (znp-1 antibody, white). Confocal projection. (A) Motor axons grow ventrally from the spinal cord (SC) in controls. (B,C) The PAC forms normally in controls (arrowheads). (D) Motoneurons and their axons are absent in olig2 morphants. (E,F) PAC does not form in olig2 morphants (arrowheads). (G-I) Lateral view of 55 hpf fli1a:egfpy1; hb9:KikGR after photoconversion, with vessels in green and motoneurons in white. Confocal projections. Motoneuron cell bodies were targeted by laser in one somite per embryo (red rectangle), resulting in ablation of motoneurons, (G) missing caudal primary motoneuron (CaP) axon (arrow), and (H,I) missing PAC (arrowhead) in that somite. (J) The PAC was scored in 4-5 somites per embryo in uninjected control embryos (complete, 82±6%; partial, 12±6%; absent, 6±4%) and olig2 morphants (complete, 52±5%; partial, 20±3%; absent, 28±4%). All values are mean±s.e.m.; P-value determined by Mann-Whitney U test. n, number of hemisegments (number of embryos). (K) The number of somites with PACs was quantified in somites that were untreated or successfully laser ablated. Untreated: complete or partial, 95%; absent, 5%. Ablated: complete or partial, 50%; absent, 50%. n, number of hemisegments; P-value determined by Fischer′s exact test. A, anterior; D, dorsal. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg/+ standard conditions Long-pec vasculature IHC
y1Tg/+ + MO1-olig2 standard conditions Long-pec vasculature IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
znp-1 IHC y1Tg/+ standard conditions Long-pec motor neuron
IHC Long-pec motor neuron axon
IHC y1Tg/+ + MO1-olig2 standard conditions Long-pec motor neuron
IHC Long-pec Not Detected motor neuron axon
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg/+ + MO1-olig2 standard conditions Long-pec motor neuron lacks parts or has fewer parts of type motor neuron axon, abnormal
Long-pec parachordal vessel immature, abnormal
Long-pec parachordal vessel venous endothelial cell migration involved in lymph vessel development arrested, abnormal
Acknowledgments:
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