ZFIN ID: ZDB-FIG-110812-40
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 6

RoP axons and associated secondary axons at the HMS do not form in netrin 1a and dcc morphants. (A) Diagram of RoP, middle primary (MiP) and caudal primary (CaP) axons at 36 hpf. (B-D) Lateral view of 55 hpf hb9:GFP zebrafish embryos injected with (B) no MO, (C) netrin 1a splice-blocking MO (netrin1aSBMO) or (D) dcc translation-blocking MO (dccTBMO). Confocal projections. (B) Uninjected controls show axons at the HMS in almost every somite (arrowheads). In embryos injected with (C) netrin1aSBMO or (D) dccTBMO, axons fail to form at the HMS (arrowheads). (E) Axons at the HMS were counted in 3-4 somites per embryo in segments 7-11 in control, netrin1aSBMO- and dccTBMO-injected embryos. Uninjected, 97±3%; netrin 1a morphant, 27±7%. Uninjected, 98±2%; dcc morphant, 49±7%. All values are mean±s.e.m.; P-value determined by Mann-Whitney U test. n, number of hemisegments (number of embryos). A, anterior; D, dorsal; L, lateral; RoP, rostral primary motoneuron; MiP, middle primary motoneuron; CaP, caudal primary motoneuron. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP ml2Tg standard conditions Long-pec CaP motoneuron IFL
Long-pec CaP motoneuron axon IFL
Long-pec MiP motor neuron IFL
Long-pec MiP motor neuron axon IFL
Long-pec RoP motor neuron IFL
Long-pec RoP motor neuron axon IFL
ml2Tg + MO1-dcc standard conditions Long-pec CaP motoneuron IFL
Long-pec CaP motoneuron axon IFL
Long-pec MiP motor neuron IFL
Long-pec MiP motor neuron axon IFL
Long-pec RoP motor neuron IFL
Long-pec RoP motor neuron axon IFL
ml2Tg + MO2-ntn1a standard conditions Long-pec CaP motoneuron IFL
Long-pec CaP motoneuron axon IFL
Long-pec MiP motor neuron IFL
Long-pec MiP motor neuron axon IFL
Long-pec RoP motor neuron IFL
Long-pec RoP motor neuron axon IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
ml2Tg + MO1-dcc standard conditions Long-pec horizontal myoseptum lacks parts or has fewer parts of type motor neuron axon, abnormal
Long-pec horizontal myoseptum lacks parts or has fewer parts of type RoP motor neuron axon, abnormal
ml2Tg + MO2-ntn1a standard conditions Long-pec horizontal myoseptum lacks parts or has fewer parts of type motor neuron axon, abnormal
Long-pec horizontal myoseptum lacks parts or has fewer parts of type RoP motor neuron axon, abnormal
Acknowledgments:
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