ZFIN ID: ZDB-FIG-110812-38
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

dcc is required for turning of secondary sprouts at the HMS and injection of dcc mRNA rescues PAC formation in dcc morphants. (A-B2) 48 hpf fli1a:egfpy1 zebrafish embryos injected with (A,A2) plcg1 splice-blocking MO (plcg1SBMO) only (control) or (B,B2) plcg1SBMO plus dcc translation-blocking MO (dccTBMO). (A) Lateral view. In plcg1 morphants, secondary sprouts grow from the PCV and turn at the HMS to form the PAC (arrowhead). (A2) Transverse volume rendering of A showing mediolateral turn of secondary sprout. (star). (B) Lateral view. In embryos co-injected with plcg1SBMO and dccTBMO, secondary sprouts form but fail to turn and form the PAC (arrowheads). (B2) Transverse volume rendering of B showing failure of secondary sprout to turn laterally (star). (C) Secondary sprouts formed from the PCV were counted in 4-5 intersomitic boundaries per embryo between segments 7-11 in plcg1 single morphants (control, 97±3%) and in plcg1SBMO/dccTBMO double morphants (dccTBMO, 90±5%), and showed no significant difference (n.s.). (D) Secondary sprouts that turned to form the PAC were counted in plcg1 single morphants (87±5%) and plcg1/dcc double morphants (18±5%), which showed greatly reduced turning in the absence of Dcc. (E,F) Lateral views of 48 hpf fli1a:egfpy1 embryos injected with (E) dccTBMO or (F) dccTBMO and dcc mRNA. The PAC is absent in dcc morphants (E, arrowheads) and restored when dcc morphants are co-injected with dcc mRNA (F, arrow and arrowheads indicate complete and partial rescue, respectively). (G) Quantification of complete, partial or absent PAC. Uninjected: complete, 99±1%; partial, 1±1%; absent, 0±0%. dcc morphant: complete, 31±7%; partial, 25±6%; absent, 44±7%. dcc morphant + RNA: complete, 86±5%; partial, 9±4%; absent, 5±3%. All values are mean±s.e.m.; error bars show s.e.m.; P-value determined by Mann-Whitney U test (comparing absent PACs in G). n, number of hemisegments (number of embryos). A, anterior; D, dorsal; L, lateral; PAC, parachordal chain. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg/+ + MO1-dcc standard conditions Long-pec vasculature IFL
y1Tg/+ + MO1-dcc + MO3-plcg1 standard conditions Long-pec vasculature IFL
y1Tg/+ + MO3-plcg1 standard conditions Long-pec vasculature IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg/+ + MO1-dcc standard conditions Long-pec branching involved in lymph vessel morphogenesis process quality, abnormal
Long-pec lymph vessel development process quality, abnormal
Long-pec parachordal vessel immature, abnormal
Long-pec parachordal vessel venous endothelial cell migration involved in lymph vessel development arrested, abnormal
y1Tg/+ + MO1-dcc + MO3-plcg1 standard conditions Long-pec branching involved in lymph vessel morphogenesis process quality, abnormal
Long-pec parachordal vessel immature, abnormal
Long-pec parachordal vessel venous endothelial cell migration involved in lymph vessel development arrested, abnormal
Acknowledgments:
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