ZFIN ID: ZDB-FIG-110812-36
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Anatomical Terms:
Stage Range: 26+ somites to Long-pec
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Long-pec

Fig. 2

Muscle pioneers express netrin 1a along the HMS prior to and during PAC formation and are required for PAC formation. (A-B2) mRNA in situ hybridization (ISH) shows netrin 1a expression in MPs and neural tube (NT) at 22 and 32 hpf. (B2) Transverse section of zebrafish embryo in B at the level indicated. (C-H) The muscle pioneers (MPs) are the critical source of Netrin 1a for PAC formation. Treatment with 50 µM cyclopamine prevents nearly all MP formation and leads to failure of PAC formation. 48 hpf fli1a:egfpy1 embryos antibody stained for vessels (GFP, green) and MPs (4D9 anti-engrailed, magenta). Confocal projections. (C-E) In DMSO-treated control embryos, MPs are present at the HMS (D) and the PAC forms normally (C, arrowheads). (F-H) In cyclopamine-treated embryos, very few MPs form (G) and the PAC is missing (F, arrowheads). (I) PAC formation was assessed in hemisegments 7-11 in DMSO-treated (97±2% complete; 3±2% partial; 0±0% absent) and cyclopamine-treated (21±6% complete; 15±4% partial; 64±7% absent) embryos. All values are mean±s.e.m.; P-value determined by Mann-Whitney U test comparing absent PACs. n, number of hemisegments (number of embryos). A, anterior; D, dorsal; NT, neural tube. Scale bars: 200 μm in A-B2 50 μm in C-H.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP y1Tg/+ standard conditions Long-pec vasculature IFL
y1Tg/+ chemical treatment: Cyclopamine Long-pec vasculature IFL
ntn1a WT standard conditions 26+ somites muscle pioneer ISH
26+ somites neural tube ISH
Prim-15 muscle pioneer ISH
Prim-15 spinal cord ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC y1Tg/+ standard conditions Long-pec muscle pioneer
IHC y1Tg/+ chemical treatment: Cyclopamine Long-pec muscle pioneer
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg/+ chemical treatment: Cyclopamine Long-pec muscle pioneer decreased amount, abnormal
Long-pec parachordal vessel immature, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development