PUBLICATION

Motoneurons are essential for vascular pathfinding

Authors
Lim, A.H., Suli, A., Yaniv, K., Weinstein, B., Li, D.Y., and Chien, C.B.
ID
ZDB-PUB-110811-31
Date
2011
Source
Development (Cambridge, England)   138(17): 3847-3857 (Journal)
Registered Authors
Chien, Chi-Bin, Lim, Amy, Suli, Arminda, Weinstein, Brant M., Yaniv, Karina
Keywords
netrin, DCC, lymphatic, parachordal chain, motoneuron, axon guidance, vascular guidance, angiogenesis, zebrafish
MeSH Terms
  • Animals
  • In Situ Hybridization
  • Motor Neurons/cytology
  • Motor Neurons/metabolism*
  • Nerve Growth Factors/genetics
  • Nerve Growth Factors/metabolism*
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
21828101 Full text @ Development
Abstract

The neural and vascular systems share common guidance cues that have direct and independent signaling effects on nerves and endothelial cells. Here, we show that zebrafish Netrin 1a directs Dcc-mediated axon guidance of motoneurons and that this neural guidance function is essential for lymphangiogenesis. Specifically, Netrin 1a secreted by the muscle pioneers at the horizontal myoseptum (HMS) is required for the sprouting of dcc-expressing rostral primary motoneuron (RoP) axons and neighboring axons along the HMS, adjacent to the future trajectory of the parachordal chain (PAC). These axons are required for the formation of the PAC and, subsequently, the thoracic duct. The failure to form the PAC in netrin 1a or dcc morphants is phenocopied by laser ablation of motoneurons and is rescued both by cellular transplants and overexpression of dcc mRNA. These results provide a definitive example of the requirement of axons in endothelial guidance leading to the parallel patterning of nerves and vessels in vivo.

Genes / Markers
Figures
Figure Gallery
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes