ZFIN ID: ZDB-FIG-110812-39
Lim et al., 2011 - Motoneurons are essential for vascular pathfinding. Development (Cambridge, England)   138(17):3847-3857 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec

Fig. 5

dcc is not detected in endothelial secondary sprouts but is expressed in motoneurons with axons present at the HMS prior to PAC formation. (A,B) dcc mRNA is expressed in the ventral spinal cord at 22 and 32 hpf and is not detectable in the secondary sprouts or other trunk vasculature. (C) Whole-mount and longitudinal section of hb9:GFP transgenic zebrafish embryo showing dcc mRNA ISH (purple), where hb9:GFP labels only motoneurons at early stages (green). (D) Lateral volume rendering of fli1a:dsRedEx; hb9:GFP embryo at 55 hpf. Double transgenic labels endothelial cells (green) and motoneuron axons (white) in the trunk. Rostral primary motoneuron (RoP, yellow) axons are present at the HMS adjacent to the PAC. (D2) Outline of motoneurons alone. CaP, caudal primary motoneuron (red); MiP, middle primary motoneuron (orange; axon omitted for clarity). SC, spinal cord; A, anterior; D, dorsal. Scale bars: 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dcc WT standard conditions 26+ somites spinal cord neural tube ventral region ISH
Prim-15 spinal cord ventral region ISH
ml2Tg standard conditions 20-25 somites CaP motoneuron ISH
20-25 somites MiP motor neuron ISH
20-25 somites motor neuron ISH
20-25 somites RoP motor neuron ISH
DsRedx ml2Tg; um13Tg standard conditions Long-pec vasculature IFL
GFP ml2Tg standard conditions 20-25 somites CaP motoneuron IHC
20-25 somites MiP motor neuron IHC
20-25 somites motor neuron IHC
20-25 somites RoP motor neuron IHC
ml2Tg; um13Tg standard conditions Long-pec CaP motoneuron IFL
Long-pec MiP motor neuron IFL
Long-pec motor neuron IFL
Long-pec RoP motor neuron IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development