ZFIN ID: ZDB-FIG-090223-17
Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations. Development (Cambridge, England)   136(3):403-414 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to 14-19 somites

Fig. 1 Myf5 or Myod are required for slow fibre formation. Dorsal flatmounts of zebrafish embryos injected with the indicated MOs and uninjected controls (con), analysed at the 13- to 15-somite stage by whole-mount immunohistochemistry for MyHC (red) or in situ mRNA hybridisation for the indicated mRNAs (blue). Anterior to top. (Left panels) myf5+myod MOs ablated all slow myosin heavy chain (smyhc1 and slow MyHC, arrowheads), but did not completely prevent actin or tpma expression (arrowheads). myod MO slightly delayed smyhc mRNA and MyHC accumulation (arrows), whereas other single MOs had little effect. (Right panels) Single MRF MOs had no effect on adaxial expression of the other MRFs. However, the myod MO reduced myog signal in fast muscle precursors (white arrows). The box region is shown magnified beneath. Note the increase in signal for each MRF caused by its cognate MO (asterisks). s, somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta1b WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
myf5 WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
myf6 WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
myod1 WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites segmental plate ISH
10-13 somites to 14-19 somites somite ISH
myog WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
smyhc1 WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 10-13 somites to 14-19 somites Not Detected adaxial cell ISH
10-13 somites to 14-19 somites Not Detected somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
tpma WT control 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO1-myog standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
WT + MO3-myf5 standard conditions 10-13 somites to 14-19 somites adaxial cell ISH
10-13 somites to 14-19 somites somite ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development