ZFIN ID: ZDB-FIG-090223-23
Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations. Development (Cambridge, England)   136(3):403-414 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 7 Residual fast fibres after Myf5 plus Myod knockdown are hedgehog dependent. In situ mRNA hybridisation (A,B) and immunofluorescent confocal stacks (C) of 23 ss, 24-26 hpf wild-type, Tg(mylz2:GFP) or myf5+/hu2022 incross embryos. Lateral views, anterior to left. (A) Cyclopamine (CyA) treatment of embryos from a myf5+/hu2022 incross injected with myod MO ablates residual myhz1 expression (arrows, magnified in insets). Fast muscle still forms in the absence of Myod and Hh signals. Note the altered somite shape, paralleling the absence of slow fibres. (B) Expression of eng1a and eng2a (arrowheads) is missing from myf5hu2022 mutants injected with myod MO and is reduced in myod+myog double morphants. Eng1a is not affected by myf5 mutation (revealed by weak myf5 mRNA expression) or myod MO alone, although eng2a is drastically reduced in the myod morphant. (C) Tg(mylz2:GFP) embryos injected with myod MO show reduction of both the weak Engrailed protein in MFF nuclei (yellow arrowheads) and the strong Engrailed expression of muscle pioneers (blue arrowheads). (D) Schematic illustrating how the cells generated in medial and lateral modes of myogenesis in the early zebrafish somite (left) each contribute to several later compartments (right). Note that dermomyotomal contributions are not defined because cell lineage is largely unknown.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
en1a WT control 20-25 somites somite ISH
WT + MO1-myod1 standard conditions Prim-5 somite ISH
WT + MO1-myod1 + MO1-myog standard conditions 20-25 somites somite ISH
myf5hu2022/hu2022 control Prim-5 somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions Prim-5 Not Detected somite ISH
en2a WT + MO1-myod1 standard conditions 20-25 somites somite ISH
WT + MO1-myod1 + MO1-myog standard conditions 20-25 somites Not Detected somite ISH
myf5hu2022/hu2022 control 20-25 somites somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 20-25 somites Not Detected somite ISH
GFP i135Tg control Prim-5 somite IFL
i135Tg + MO1-myod1 standard conditions Prim-5 somite IFL
myf5 WT control 20-25 somites somite ISH
WT + MO1-myod1 standard conditions 20-25 somites somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 20-25 somites somite ISH
myhz1.1 WT + MO1-myod1 chemical treatment: Cyclopamine Prim-5 fast muscle cell ISH
WT + MO1-myod1 standard conditions Prim-5 fast muscle cell ISH
myf5hu2022/hu2022 chemical treatment: Cyclopamine Prim-5 fast muscle cell ISH
myf5hu2022/hu2022 + MO1-myod1 chemical treatment: Cyclopamine Prim-5 Not Detected fast muscle cell ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions Prim-5 fast muscle cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-eng IHC i135Tg control Prim-5 fast muscle cell
IHC Prim-5 muscle pioneer
IHC i135Tg + MO1-myod1 standard conditions Prim-5 muscle pioneer
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development