ZFIN ID: ZDB-FIG-090223-19
Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations. Development (Cambridge, England)   136(3):403-414 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Myf5 requirement differs between somitic, head and fin muscle development. In situ mRNA hybridisation and/or MyHC immunohistochemistry of control and morphant wild-type or myf5+/hu2022 incross embryos. Dorsal flatmounts with anterior to top (A,F), or whole-mounts with anterior to left in ventral (B, upper row, C,D), lateral (B, lower row) or dorsal (E) view. (A) myf5hu2022 mutants injected with myod MO show a loss of the adaxial slow muscle differentiation markers smyhc1, actin, tpma and mef2ca and MyHC immunostaining (black arrows), but retain other adaxial markers such as prdm1 and ptc1 (white arrows). (B-D) Head muscles are present in 80 hpf myf5hu2022 mutants but are missing from embryos injected with myod (B,C) or myog MO (C). Arrowheads indicate lack of ventral (green), dorsal (yellow), extra-ocular (red) and sternohyoideus (sh, white) muscles. All embryos show normal heart (h, blue arrow). Note the presence of sternohyoideus in myog morphants. All embryos from a myf5+/hu2022 incross have normal expression of myhz1 at 72 hpf (C) and myog at 48 hpf (D) in the head. myod morphants lack myog mRNA (19/21), although sternohyoideus was still detected in some embryos (6/21). (E) In pectoral fin, myog mRNA in muscle masses (pink arrowheads) and MyHC are ablated or reduced by loss of Myod, but not by loss of Myf5. Ibx2 mRNA is unaffected by myod MO. (F) Expression of hsp90a is delayed in fast muscle precursors (arrow) of the myf5hu2022 mutant and recovers in somites (arrowhead), but is drastically reduced throughout the axis in myf5hu2022 mutants injected with myod MO, both in slow and fast precursors. meox1 expression is unchanged in fast precursors.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
acta1b WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
14-19 somites segmental plate ISH
14-19 somites somite ISH
myf5hu2022/+ + MO1-myod1 standard conditions 14-19 somites Not Detected adaxial cell ISH
hsp90aa1.1 WT standard conditions 14-19 somites fast muscle myoblast ISH
14-19 somites slow muscle myoblast ISH
14-19 somites somite ISH
WT + MO1-myod1 standard conditions 14-19 somites fast muscle myoblast ISH
14-19 somites slow muscle myoblast ISH
14-19 somites somite ISH
myf5hu2022/hu2022 standard conditions 14-19 somites fast muscle myoblast ISH
14-19 somites slow muscle myoblast ISH
14-19 somites somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 14-19 somites fast muscle myoblast ISH
14-19 somites slow muscle myoblast ISH
14-19 somites somite ISH
lbx2 WT standard conditions Protruding-mouth pectoral fin musculature ISH
WT + MO1-myod1 standard conditions Protruding-mouth pectoral fin musculature ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions Protruding-mouth pectoral fin musculature ISH
mef2ca WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 14-19 somites Not Detected adaxial cell ISH
meox1 WT standard conditions 14-19 somites fast muscle myoblast ISH
WT + MO1-myod1 standard conditions 14-19 somites fast muscle myoblast ISH
myf5hu2022/hu2022 standard conditions 14-19 somites fast muscle myoblast ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 14-19 somites fast muscle myoblast ISH
myf5 WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
14-19 somites segmental plate ISH
14-19 somites somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
14-19 somites segmental plate ISH
14-19 somites somite ISH
myhz1.1 WT standard conditions Protruding-mouth cephalic musculature ISH
WT + MO1-myod1 standard conditions Protruding-mouth Not Detected cephalic musculature ISH
WT + MO1-myog standard conditions Protruding-mouth Not Detected cephalic musculature ISH
Protruding-mouth sternohyoid ISH
myf5hu2022/hu2022 standard conditions Protruding-mouth cephalic musculature ISH
myog WT standard conditions Long-pec cephalic musculature ISH
Long-pec pectoral fin musculature ISH
WT + MO1-myod1 standard conditions Long-pec Not Detected cephalic musculature ISH
Long-pec Not Detected pectoral fin musculature ISH
myf5hu2022/hu2022 standard conditions Long-pec cephalic musculature ISH
Long-pec pectoral fin musculature ISH
prdm1a WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
myf5hu2022/+ + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
ptch2 WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
myf5hu2022/+ + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
smyhc1 WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
14-19 somites somite ISH
myf5hu2022/+ + MO1-myod1 standard conditions 14-19 somites Not Detected adaxial cell ISH
14-19 somites Not Detected somite ISH
tpma WT + MO1-myod1 standard conditions 14-19 somites adaxial cell ISH
14-19 somites somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 14-19 somites Not Detected adaxial cell ISH
14-19 somites Not Detected somite ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT standard conditions Protruding-mouth cephalic musculature
IHC Protruding-mouth heart
IHC Protruding-mouth pectoral fin musculature
IHC WT + MO1-myod1 standard conditions Protruding-mouth Not Detected cephalic musculature
IHC Protruding-mouth heart
IHC Protruding-mouth Not Detected pectoral fin musculature
IHC myf5hu2022/hu2022 standard conditions Protruding-mouth cephalic musculature
IHC Protruding-mouth heart
IHC Protruding-mouth pectoral fin musculature
IHC myf5hu2022/hu2022 + MO1-myod1 standard conditions Protruding-mouth Not Detected cephalic musculature
IHC Protruding-mouth heart
IHC Protruding-mouth Not Detected pectoral fin musculature
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 standard conditions Protruding-mouth cephalic musculature aplastic, abnormal
WT + MO1-myog standard conditions Protruding-mouth cephalic musculature aplastic, abnormal
Protruding-mouth sternohyoid present, normal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development