ZFIN ID: ZDB-FIG-090223-21
Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations. Development (Cambridge, England)   136(3):403-414 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-5

Fig. 5 Mrf4 has no role in trunk muscle differentiation. (A) Schematic illustrating the location of the mutation encoded by mrf4hu2041. (B) Proportion of mrf4hu2041/hu2041 (red), mrf4hu2041/+ (green) or mrf4+/+ (blue) in progeny from heterozygote crosses. The number of embryos of each genotype is shown. Homozygote mutants survive well and without obvious defects. (C) Whole-mount in situ mRNA hybridisation of myf5+myod morphants or myod MO-injected myf5hu2022 mutants reveal that residual medial fast fibres express myog (red) but not mrf4 (purple) at 22 ss, but accumulate mrf4 mRNA after terminal differentiation at 24 hpf. Lateral view, anterior to left. (D) In situ mRNA hybridisation for myhz1 (purple) in embryos from a mrf4+/hu2041 incross reveals that injection of myf5+myod MOs fails to ablate all muscle irrespective of mrf4 genotype.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myf6 WT control 20-25 somites fast muscle cell ISH
WT + MO1-myod1 standard conditions 20-25 somites fast muscle cell ISH
Prim-5 fast muscle cell ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 20-25 somites Not Detected fast muscle cell ISH
myf5hu2022/hu2022 standard conditions 20-25 somites fast muscle cell ISH
Prim-5 fast muscle cell ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 20-25 somites Not Detected fast muscle cell ISH
Prim-5 fast muscle cell ISH
myhz1.1 WT control Prim-5 somite ISH
myf6hu2041/hu2041 standard conditions Prim-5 somite ISH
myf6hu2041/hu2041 + MO1-myod1 + MO3-myf5 standard conditions Prim-5 muscle cell ISH
myog WT control 20-25 somites somite ISH
WT + MO1-myod1 standard conditions 20-25 somites somite ISH
Prim-5 somite ISH
WT + MO1-myod1 + MO3-myf5 standard conditions 20-25 somites fast muscle cell ISH
myf5hu2022/hu2022 standard conditions 20-25 somites somite ISH
Prim-5 somite ISH
myf5hu2022/hu2022 + MO1-myod1 standard conditions 20-25 somites fast muscle cell ISH
Prim-5 fast muscle cell ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development