ZFIN ID: ZDB-FIG-090223-22
Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations. Development (Cambridge, England)   136(3):403-414 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6 Loss of Myod and Myog ablates somitic fast muscle. In situ mRNA hybridisation (A,B,E) or immunofluorescent confocal stacks (C,D) of 24 hpf wild-type (con), Tg(acta1:GFP) or myf5+/hu2022 incrosses injected with the indicated MOs. Lateral views, anterior to left. (A) Magnified regions (boxed) showing that lack of Myf5, Myod and Myog results in loss of all muscle. Knockdown of Myod and Myog results in loss of most fast, but not slow, muscle. Note that mylz2 (red) is weakly expressed in immature slow muscle. (B) Loss of Myog and Myf5 has little effect, if any, on fast myhz1 or slow smyhc1 expression. (C) Injection of myod+myog MOs into Tg(acta1:GFP) fish results in loss of fast muscle, as shown by loss of Mef2 staining and GFP medial to the slow fibre layer accompanied by somitic apoptosis as revealed with DAPI (blue in the optical transverse sections). Elongated mononucleate slow fibres differentiate and express GFP, slow MyHC and Mef2. myod morphants confirm the lateral reduction of fast muscle mass. (D) myod+myog MOs ablate fast MyHC in somites, whereas single MOs have a lesser effect. (E) Titration of myod+myog MOs progressively diminishes residual myhz1-expressing cells. The lower panels are high-magnification images of mid-trunk somites (boxed).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
GFP zf13Tg control Prim-5 muscle cell IFL
zf13Tg + MO1-myod1 standard conditions Prim-5 muscle cell IFL
zf13Tg + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected fast muscle cell IFL
Prim-5 muscle cell IFL
myhz1.1 WT control Prim-5 fast muscle cell ISH
WT + MO1-myod1 + MO1-myog standard conditions Prim-5 fast muscle cell ISH
WT + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected fast muscle cell ISH
myf5hu2022/hu2022 standard conditions Prim-5 fast muscle cell ISH
myf5hu2022/hu2022 + MO1-myog standard conditions Prim-5 fast muscle cell ISH
mylpfa WT control Prim-5 fast muscle cell ISH
Prim-5 Not Detected slow muscle myoblast ISH
WT + MO1-myod1 + MO1-myog standard conditions Prim-5 fast muscle cell ISH
Prim-5 Not Detected slow muscle myoblast ISH
myf5hu2022/hu2022 + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected fast muscle cell ISH
Prim-5 Not Detected slow muscle myoblast ISH
smyhc1 WT control Prim-5 slow muscle cell ISH
WT + MO1-myod1 + MO3-myf5 standard conditions Prim-5 slow muscle cell ISH
myf5hu2022/hu2022 standard conditions Prim-5 slow muscle cell ISH
myf5hu2022/hu2022 + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected slow muscle cell ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-EB165 IHC zf13Tg control Prim-5 somite fast muscle cell
IHC zf13Tg + MO1-myod1 standard conditions Prim-5 somite fast muscle cell
IHC zf13Tg + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected somite fast muscle cell
IHC zf13Tg + MO1-myog standard conditions Prim-5 somite fast muscle cell
Ab-F59 IHC zf13Tg control Prim-5 slow muscle cell
IHC zf13Tg + MO1-myod1 standard conditions Prim-5 slow muscle cell
IHC zf13Tg + MO1-myod1 + MO1-myog standard conditions Prim-5 slow muscle cell
Ab1-mef2 IHC zf13Tg control Prim-5 muscle cell
IHC zf13Tg + MO1-myod1 standard conditions Prim-5 muscle cell
IHC zf13Tg + MO1-myod1 + MO1-myog standard conditions Prim-5 Not Detected fast muscle cell
IHC Prim-5 muscle cell
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-myod1 + MO1-myog standard conditions Prim-5 fast muscle cell absent, abnormal
myf5hu2022/hu2022 + MO1-myod1 + MO1-myog standard conditions Prim-5 muscle aplastic, abnormal
zf13Tg + MO1-myod1 + MO1-myog standard conditions Prim-5 fast muscle cell absent, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development