PUBLICATION
Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone
- Authors
- Smith, C.J., Morris, A.D., Welsh, T.G., Kucenas, S.
- ID
- ZDB-PUB-141001-1
- Date
- 2014
- Source
- PLoS Biology 12: e1001961 (Journal)
- Registered Authors
- Kucenas, Sarah, Morris, Angie, Smith, Cody
- Keywords
- Spinal cord, Embryos, Axons, Motor system, Central nervous system, Neural crest, Schwann cells, Zebrafish
- MeSH Terms
-
- Axons/ultrastructure
- Animals
- Motor Neurons/cytology*
- Motor Neurons/metabolism
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/metabolism
- Stem Cells/cytology*
- Stem Cells/metabolism
- Oligodendroglia/cytology*
- Oligodendroglia/metabolism
- Schwann Cells/cytology
- Schwann Cells/metabolism
- Gene Expression Regulation, Developmental
- Cell Movement
- Spinal Cord/cytology*
- Spinal Cord/growth & development
- Spinal Cord/metabolism
- Cell Lineage/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Repressor Proteins/genetics
- Repressor Proteins/metabolism
- Cell Differentiation
- Zebrafish/genetics
- Zebrafish/growth & development*
- Zebrafish/metabolism
- Organ Specificity
- Myelin Sheath/ultrastructure
- Morphogenesis
- Time-Lapse Imaging
- PubMed
- 25268888 Full text @ PLoS Biol.
Citation
Smith, C.J., Morris, A.D., Welsh, T.G., Kucenas, S. (2014) Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone. PLoS Biology. 12:e1001961.
Abstract
Rapid conduction of action potentials along motor axons requires that oligodendrocytes and Schwann cells myelinate distinct central and peripheral nervous system (CNS and PNS) domains along the same axon. Despite the importance of this arrangement for nervous system function, the mechanisms that establish and maintain this precise glial segregation at the motor exit point (MEP) transition zone are unknown. Using in vivo time-lapse imaging in zebrafish, we observed that prior to myelination, oligodendrocyte progenitor cells (OPCs) extend processes into the periphery via the MEP and immediately upon contact with spinal motor root glia retract back into the spinal cord. Characterization of the peripheral cell responsible for repelling OPC processes revealed that it was a novel, CNS-derived population of glia we propose calling MEP glia. Ablation of MEP glia resulted in the absence of myelinating glia along spinal motor root axons and an immediate breach of the MEP by OPCs. Taken together, our results identify a novel population of CNS-derived peripheral glia located at the MEP that selectively restrict the migration of OPCs into the periphery via contact-mediated inhibition.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping