PUBLICATION

Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone

Authors
Smith, C.J., Morris, A.D., Welsh, T.G., Kucenas, S.
ID
ZDB-PUB-141001-1
Date
2014
Source
PLoS Biology   12: e1001961 (Journal)
Registered Authors
Kucenas, Sarah, Morris, Angie, Smith, Cody
Keywords
Spinal cord, Embryos, Axons, Motor system, Central nervous system, Neural crest, Schwann cells, Zebrafish
MeSH Terms
  • Myelin Sheath/ultrastructure
  • Axons/ultrastructure
  • Organ Specificity
  • Cell Differentiation
  • Gene Expression Regulation, Developmental
  • Cell Movement
  • Animals
  • Schwann Cells/cytology
  • Schwann Cells/metabolism
  • Repressor Proteins/genetics
  • Repressor Proteins/metabolism
  • Morphogenesis
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/metabolism
  • Oligodendroglia/cytology*
  • Oligodendroglia/metabolism
  • Motor Neurons/cytology*
  • Motor Neurons/metabolism
  • Cell Lineage/genetics
  • Spinal Cord/cytology*
  • Spinal Cord/growth & development
  • Spinal Cord/metabolism
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Time-Lapse Imaging
(all 30)
PubMed
25268888 Full text @ PLoS Biol.
Abstract
Rapid conduction of action potentials along motor axons requires that oligodendrocytes and Schwann cells myelinate distinct central and peripheral nervous system (CNS and PNS) domains along the same axon. Despite the importance of this arrangement for nervous system function, the mechanisms that establish and maintain this precise glial segregation at the motor exit point (MEP) transition zone are unknown. Using in vivo time-lapse imaging in zebrafish, we observed that prior to myelination, oligodendrocyte progenitor cells (OPCs) extend processes into the periphery via the MEP and immediately upon contact with spinal motor root glia retract back into the spinal cord. Characterization of the peripheral cell responsible for repelling OPC processes revealed that it was a novel, CNS-derived population of glia we propose calling MEP glia. Ablation of MEP glia resulted in the absence of myelinating glia along spinal motor root axons and an immediate breach of the MEP by OPCs. Taken together, our results identify a novel population of CNS-derived peripheral glia located at the MEP that selectively restrict the migration of OPCs into the periphery via contact-mediated inhibition.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
ABchemical treatment: DAPTProtruding-mouth
ABchemical treatment: DAPTDay 4
erbb3bst48/st48standard conditionsLong-pec
erbb3bst48/st48; vu17Tg; vu234Tgchemical treatment: N-phenylthioureaLong-pec
1 - 4 of 4
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ct110aRGtTransgenic Insertion
mi2001TgTransgenic Insertion
    nl1TgTransgenic Insertion
      sl3TgTransgenic Insertion
        st48
          		Point Mutation
          st61
            		Point Mutation
            ue2TgTransgenic Insertion
              vu17TgTransgenic Insertion
                vu19TgTransgenic Insertion
                  vu234TgTransgenic Insertion
                    1 - 10 of 14
                    Show
                    Human Disease / Model
                    No data available
                    Sequence Targeting Reagents
                    Target Reagent Reagent Type
                    nkx2.2aMO4-nkx2.2aMRPHLNO
                    olig2MO4-olig2MRPHLNO
                    1 - 2 of 2
                    Show
                    Fish
                    Fish
                    AB
                    erbb2st61/st61
                    erbb3bst48/st48
                    erbb3bst48/st48; vu17Tg; vu234Tg
                    erbb3bst48/st48; vu234Tg
                    foxd3ct110aRGt/ct110aRGt
                    foxd3ct110aRGt; mi2001Tg
                    foxd3ct110aRGt; ue2Tg
                    mi2001Tg
                    nl1Tg
                    1 - 10 of 22
                    Show
                    Antibodies
                    Name Type Antigen Genes Isotypes Host Organism
                    Ab1-mbppolyclonalRabbit
                    Ab2-sox10polyclonalRabbit
                    1 - 2 of 2
                    Show
                    Orthology
                    No data available
                    Engineered Foreign Genes
                    Marker Marker Type Name
                    DsRedEFGDsRed
                    DsRed2EFGDsRed2
                    EGFPEFGEGFP
                    EosEFGEos
                    GFPEFGGFP
                    mCherryEFGmCherry
                    mRFPEFGmRFP
                    1 - 7 of 7
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                    Mapping
                    No data available
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                    Citation
                    Smith, C.J., Morris, A.D., Welsh, T.G., Kucenas, S. (2014) Contact-Mediated Inhibition Between Oligodendrocyte Progenitor Cells and Motor Exit Point Glia Establishes the Spinal Cord Transition Zone. PLoS Biology. 12:e1001961.