ZFIN ID: ZDB-FIG-181024-9
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 5

Fox genes are required for chondrogenic gene expression downstream of Sox9a. (A) Fluorescence in situ hybridizations show that barx1+ pre-cartilage condensations of the first two arches are unaltered in foxc1a; foxc1b double mutants (c1a−/−; c1b−/−) and foxf1; foxf2a; foxf2b triple mutants (f1−/−; f2a−/−; f2b−/−) compared with sibling controls. (B,C) Schematic of the cartilaginous facial skeleton shows regions examined by fluorescence in situ hybridization. Maximum intensity projections for foxc1a; foxc1b mutants at 56 hpf (B) and representative sections for foxf1; foxf2a; foxf2b mutants at 72 hpf (C) show expression of the chondrogenic genes col2a1a, matn1, matn4 or acana (green) relative to sox9a (red). Arrows indicate regions in which sox9a+ cells have reduced chondrogenic gene expression relative to sibling controls. Numbers denote proportions of embryos with displayed patterns. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
acana WT control Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
Protruding-mouth Meckel's cartilage ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage ISH
barx1 WT control Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
col2a1a WT control Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
Protruding-mouth Meckel's cartilage ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage ISH
matn1 WT control Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
Protruding-mouth Meckel's cartilage ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage ISH
matn4 WT control Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
Protruding-mouth Meckel's cartilage ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage ISH
sox9a WT standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
Protruding-mouth Meckel's cartilage ISH
foxc1ael543/+; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxc1ael543/el543; foxc1bel620/el620 standard conditions Long-pec hyosymplectic cartilage acana expression decreased distribution, abnormal
Long-pec hyosymplectic cartilage col2a1a expression decreased distribution, abnormal
Long-pec hyosymplectic cartilage matn1 expression decreased amount, abnormal
Long-pec hyosymplectic cartilage matn1 expression decreased distribution, abnormal
Long-pec hyosymplectic cartilage matn4 expression decreased distribution, abnormal
Long-pec palatoquadrate cartilage acana expression decreased distribution, abnormal
Long-pec palatoquadrate cartilage col2a1a expression decreased distribution, abnormal
Long-pec palatoquadrate cartilage matn1 expression decreased amount, abnormal
Long-pec palatoquadrate cartilage matn1 expression decreased distribution, abnormal
Long-pec palatoquadrate cartilage matn4 expression decreased distribution, abnormal
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Protruding-mouth Meckel's cartilage acana expression decreased distribution, abnormal
Protruding-mouth Meckel's cartilage col2a1a expression decreased distribution, abnormal
Protruding-mouth Meckel's cartilage matn1 expression decreased distribution, abnormal
Protruding-mouth Meckel's cartilage matn4 expression decreased distribution, abnormal
Acknowledgments:
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