ZFIN ID: ZDB-FIG-181024-6
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 2

Loss of upper facial cartilage in Fox-C mutants. (A) Ventral views of dissected facial skeletons, with cartilage in blue and bones and teeth in red. Schematics show affected elements in color. Ch, ceratohyal; Hm, hyomandibular; M, Meckel's; Pq, palatoquadrate; Sy, symplectic. (B) Lateral views of intact heads stained with Alcian Blue (cartilage) and Alizarin Red (bones and teeth). Shown below are unilateral dissections of skeletal elements of the first and second arches (red boxed region). Accompanying schematics show dose-dependent reductions of the Pq (magenta), Sy (green) and Hm (blue) cartilages. foxc1a−/− and foxc1a−/−; foxc1b−/− mutants also display cardiac edema and an overall smaller head. (C) Quantification of the relative length of Sy compared with Ch. ***P<0.001 versus wild-type sibling control using Student's t-test. n.s., not significant versus control (P=0.35). Error bars represent s.e.m. (D) Dissections of neurocranial cartilages show loss of the trabecular cartilages (Tr, arrow) in foxc1a−/−; foxc1b−/− mutants. Numbers denote proportions of embryos with displayed phenotypes. Ep, ethmoid plate. Scale bars: 25 μm.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxc1ael543/+; foxc1bel620/el620 standard conditions Day 5 symplectic decreased size, abnormal
foxc1ael543/el543 standard conditions Day 5 head decreased size, abnormal
Day 5 hyomandibula decreased size, abnormal
Day 5 palatoquadrate cartilage decreased size, abnormal
Day 5 pericardium edematous, abnormal
Day 5 pharyngeal arch 1 cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch 2 cartilage development decreased occurrence, abnormal
Day 5 symplectic decreased size, abnormal
Days 7-13 whole organism dead, abnormal
foxc1ael543/el543; foxc1bel620/+ standard conditions Day 5 palatoquadrate cartilage decreased size, abnormal
Day 5 symplectic absent, abnormal
foxc1ael543/el543; foxc1bel620/el620 standard conditions Day 5 head decreased size, abnormal
Day 5 hyomandibula decreased size, abnormal
Day 5 neurocranial trabecula decreased size, abnormal
Day 5 neurocranium cartilage development decreased occurrence, abnormal
Day 5 palatoquadrate cartilage decreased size, abnormal
Day 5 pharyngeal arch 1 cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch 2 cartilage development decreased occurrence, abnormal
Day 5 symplectic absent, abnormal
Acknowledgments:
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