ZFIN ID: ZDB-FIG-181024-8
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-25
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-25

Fig. 4

Dorsoventral gene expression, proliferation and apoptosis in Fox mutants. (A) Fluorescence in situ hybridizations of the first two arches (numbered) show expression of jag1b (dorsal), msx1a and dlx5a (intermediate), or hand2 (ventral) in green relative to dlx2a+ arch CNCs in red. Expression is largely unaffected in foxc1a; foxc1b double mutants (c1a−/−; c1b−/−) and foxf1; foxf2a; foxf2b triple mutants (f1−/−; f2a−/−; f2b−/−) compared with sibling controls, with the exception of reduced hand2 in the ventral first arch of foxf1; foxf2a; foxf2b triple mutants (arrows). (B-C′) Analysis of proliferation. Confocal sections show BrdU+ cells (red) compared with all nuclei (Hoechst, blue) in lateral views of the first two arches (numbered) of foxc1a−/−; foxc1b−/− embryos and ventral views of the first arch Meckel's cartilage domains (M) of foxf1−/−; foxf2a−/−; foxf2b−/− embryos, relative to sibling controls. Dashed lines represent the regions quantified in the accompanying graphs. (D-E′) Analysis of apoptosis. Maximum intensity projections show the number of TUNEL+ cells (green) in lateral views of the first two arches (numbered) of foxc1a−/−; foxc1b−/− embryos and ventral views of the first arch M cartilage domains of foxf1−/−; foxf2a−/−; foxf2b−/− embryos, relative to sibling controls. Dashed lines represent the regions quantified in the accompanying graphs. Error bars represent s.e.m. Scale bars: 50 μm. n.s., not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx2a WT control Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 cranial neural crest ISH
Prim-25 pharyngeal arch 2 cranial neural crest ISH
dlx5a WT control Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
hand2 WT control Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
jag1b WT control Prim-25 pharyngeal arch 2 ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 2 ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 2 ISH
msx1a WT control Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxc1ael543/el543; foxc1bel620/el620 standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 ISH
Prim-25 pharyngeal arch 2 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Prim-25 pharyngeal arch 1 hand2 expression decreased distribution, abnormal
Acknowledgments:
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