ZFIN ID: ZDB-FIG-181024-7
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 3

Loss of midline cartilage and pharyngeal teeth in Fox-F mutants. (A) Ventral views of dissected facial skeletons, with cartilage in blue and bones and teeth in red. Schematics show affected elements in color. Bb, basibranchials; Bh, basihyal; Ch, ceratohyal; M, Meckel's; Ptp, pterygoid process. (B) Lateral views of wild-type sibling control and foxf1; foxf2a; foxf2b triple mutant at 5 dpf. Mutants show anterior truncation of the head (arrow). (C) Dissections of neurocranial cartilages show loss of the trabecular cartilages (Tr, arrow) in foxf1; foxf2a; foxf2b triple mutants. Ep, ethmoid plate. (D) Sagittal sections through the tooth-forming seventh arch of a wild-type sibling control and foxf1; foxf2a; foxf2b triple mutant at 4 dpf. All teeth (3V1, 4V2 and 5V1) are missing in mutants. c, chorda; cb5, ceratobranchial 5; ov, otic vesicle. (E,F) Whole-mount in situ hybridizations show loss of dlx2b (E) and fgf3 (F) in the developing tooth regions (arrows) of foxf1; foxf2a; foxf2b triple mutants. Numbers denote proportions of embryos with displayed phenotypes or expression patterns. Scale bars: 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2b WT standard conditions Long-pec ceratobranchial 5 primary tooth ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Long-pec Not Detected ceratobranchial 5 primary tooth ISH
fgf3 WT standard conditions Long-pec ceratobranchial 5 primary tooth ISH
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Long-pec Not Detected ceratobranchial 5 primary tooth ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
foxf1el658/el658; foxf2ael616/el616 standard conditions Day 5 basibranchial morphology, abnormal
Day 5 basihyal cartilage decreased size, abnormal
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/+ standard conditions Day 5 ceratohyal cartilage decreased size, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 pterygoid process decreased size, abnormal
Day 5 whole organism has fewer parts of type ceratobranchial 5 tooth, abnormal
Day 5 whole organism lacks all parts of type basibranchial, abnormal
Day 5 whole organism lacks all parts of type basihyal cartilage, abnormal
foxf1el658/el658; foxf2ael616/el616; foxf2bel621/el621 standard conditions Long-pec ceratobranchial 5 primary tooth dlx2b expression absent, abnormal
Long-pec ceratobranchial 5 primary tooth fgf3 expression absent, abnormal
Day 4 whole organism lacks all parts of type tooth 3V, abnormal
Day 4 whole organism lacks all parts of type tooth 4V, abnormal
Day 4 whole organism lacks all parts of type tooth 5V, abnormal
Day 5 ceratohyal cartilage decreased size, abnormal
Day 5 ethmoid cartilage decreased size, abnormal
Day 5 head decreased size, abnormal
Day 5 Meckel's cartilage decreased size, abnormal
Day 5 neurocranium cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch 1 cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch 2 cartilage development decreased occurrence, abnormal
Day 5 pterygoid process decreased size, abnormal
Day 5 whole organism has fewer parts of type ceratobranchial 5 tooth, abnormal
Day 5 whole organism lacks all parts of type basibranchial, abnormal
Day 5 whole organism lacks all parts of type basihyal cartilage, abnormal
Day 5 whole organism lacks all parts of type neurocranial trabecula, abnormal
Acknowledgments:
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