ZFIN ID: ZDB-FIG-181024-10
Xu et al., 2018 - Fox proteins are modular competency factors for facial cartilage and tooth specification. Development (Cambridge, England)   145(12) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Long-pec
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 6

Fox CNC misexpression induces ectopic cartilage and inhibits dermal bone. (A) Confocal sections show expression of foxc1a or foxf1 (green) relative to sox9a (red) in sox10:GFPCAAX+ CNCs of the first two arches (anti-GFP, blue). (B) Maximum intensity projection shows fluorescence in situ hybridization using pooled probes against foxc1a, foxc1b, foxd1 and foxd2 (green) relative to sox9a (red). (C) Unilateral dissections of the first and second arch skeletons stained for cartilage (Alcian Blue) and bone (Alizarin Red). CNC misexpression of Foxc1a (fli1a:Gal4VP16; UAS:foxc1a) or Foxf1 (fli1a:Gal4VP16; UAS:foxf1) results in ectopic cartilage in the upper face (arrowheads), and loss of the opercle bone (arrows). (D) Double fluorescence in situ hybridizations of the first two arches show reduction of the osteoblast gene runx2b (red) in the future opercle bone (arrow) but no change in sox9a (green) upon misexpression of Foxc1a or Foxf1. (E) Dissections of the first and second arch skeletons and neurocranial cartilages. Misexpression of Foxc1a in CNCs (fli1a:Gal4VP16; UAS:foxc1a) rescues cartilage development in the upper face (top) and neurocranium (bottom). Scale bars: 50 μm. (F) Model of how arch-wide Fox misexpression expands the territory in which Sox9a induces cartilage (blue) at the expense of dermal bone (red).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxc1a el375Tg standard conditions Prim-25 pharyngeal arch cartilage ISH
foxf1 el375Tg standard conditions Prim-25 pharyngeal arch cartilage ISH
GFP el375Tg standard conditions Prim-25 pharyngeal arch cartilage IHC
runx2b el360Tg; el700Tg standard conditions Long-pec opercle ISH
el360Tg; el751Tg standard conditions Long-pec opercle ISH
sox9a el360Tg; el700Tg standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
el360Tg; el751Tg standard conditions Long-pec hyosymplectic cartilage ISH
Long-pec palatoquadrate cartilage ISH
el375Tg standard conditions Prim-25 pharyngeal arch cartilage ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
el360Tg; el700Tg standard conditions Long-pec opercle runx2b expression decreased amount, abnormal
Day 5 pharyngeal arch cartilage mislocalised, abnormal
Day 5 whole organism has fewer parts of type opercle, abnormal
el360Tg; el751Tg standard conditions Long-pec opercle runx2b expression decreased amount, abnormal
Day 5 pharyngeal arch cartilage mislocalised, abnormal
Day 5 whole organism has fewer parts of type opercle, abnormal
foxc1ael543/el543; foxc1bel620/el620 standard conditions Day 5 pharyngeal arch 1 cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch 2 cartilage development decreased occurrence, abnormal
Day 5 pharyngeal arch cartilage decreased size, abnormal
Day 5 pharyngeal arch cartilage morphology, abnormal
foxc1ael543/el543; foxc1bel620/el620; el360Tg; el700Tg standard conditions Day 5 pharyngeal arch cartilage morphology, ameliorated
Day 5 pharyngeal arch cartilage size, ameliorated
Acknowledgments:
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