ZFIN ID: ZDB-FIG-180723-36
Enya et al., 2018 - A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.. Disease models & mechanisms   11(8) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 6

Whole-organism gene expression analysis identifies tumor–liver crosstalk characterized by hepatic cyp7a1 and total BA. (A) Schematic representation of zebrafish dissection in our RNA-seq experiments followed by qPCR validation and genetic approaches. (B) Expression of cyp7a1 in the liver. The scores are normalized to expression of rpl13a. The data harbors 3 biological replicates, each containing 7 larvae for 5 dpf and 5 larvae for 7 dpf, respectively. Error bars represent means±s.e.m. Statistical significance was tested using Student's t-test (unpaired, one-tailed). (C-J) Representative images of whole-mount fluorescent immunohistochemistry for Cyp7a1 in the livers of the sibling controls and tumor-bearing larvae carrying Tg(fabp10a:mCherry) at 7 dpf. Cyp7a1 (C,G), mCherry (D,H) and Hoechst 33342 (E,I) images are shown. In the merged images (F,J), Cyp7a1, mCherry and Hoechst signals are shown in green, red and blue, respectively. Scale bar: 100 µm. (K) The averages for Cyp7a1 signal intensity in the liver of the sibling controls and tumor-bearing larvae at 7 dpf are shown. The number of larvae used is 14 and 11 for the sibling controls and tumor-bearing larvae, respectively (one independent experiment). Error bars represent means±s.e.m. Statistical significance was tested using Student's t-test (unpaired, one-tailed). (L) Measurement for systemic BA levels at 7 and 9 dpf. The number of larvae used is 19 (7 dpf control larvae), 19 (7 dpf tumor-bearing larvae), 22 (9 dpf control larvae) and 18 (9 dpf tumor-bearing larvae). The scores are relative index determined using bile acids as standards (see Materials and Methods). Statistical significance was tested using Student's t-test (unpaired, one-tailed). (M) Expression of a set of secreted protein-coding genes in the intestinal tumor and normal intestine. The scores are normalized to expression of rpl13a and to the sibling controls (=1). The data harbors 5 biological replicates, each containing 5 larvae. Error bars represent means±s.e.m. Data are representative of at least two independent experiments, except C-K.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cyp7a1 nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 liver RTPCR
Days 7-13 liver IHC
fgf21 nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
igfbp1a nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
il1b nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
il22 nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
insl5a nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
lepb nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Days 7-13 intestine neoplasm RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine fgf21 expression increased amount, abnormal
Days 7-13 intestine igfbp1a expression increased amount, abnormal
Days 7-13 intestine il1b expression increased amount, abnormal
Days 7-13 intestine il22 expression increased amount, abnormal
Days 7-13 intestine insl5a expression increased amount, abnormal
Days 7-13 intestine lepb expression increased amount, abnormal
Days 7-13 intestine neoplastic, abnormal
Days 7-13 liver cyp7a1 expression decreased amount, abnormal
Days 7-13 liver cyp7a1 expression decreased amount, abnormal
Days 7-13 whole organism bile alcohol decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.