ZFIN ID: ZDB-FIG-180723-33
Enya et al., 2018 - A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.. Disease models & mechanisms   11(8) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 3

pInt-Gal4 driven krasG12D expression results in intestinal epithelial tumor. (A-H) Representative images of fluorescent immunohistochemistry for Cdh1 in transversal sections of the posterior intestine of the sibling controls and tumor-bearing larvae at 5 dpf. Cdh1 (A,B), EGFP (C,D) and DAPI (E,F) images are shown. In the merged images (G,H), Cdh1, EGFP and DAPI signals are shown in red, green and blue, respectively. Scale bar: 100 µm. (I-L) Representative images of HE-stained intestinal sections of the sibling controls (I,K) and tumor-bearing larvae (J,L) at 5 dpf. Transversal and sagittal sections are shown in I,J and K,L, respectively. Scale bars: 50 µm. (M-O) qPCR analysis for mmp genes in the intestine at 9 dpf. The scores are normalized to expression of rpl13a. The data harbors 5 biological replicates, each containing the intestines from 5 larvae. Error bars represent means±s.e.m. Statistical significance was tested using Student's t-test (unpaired, one-tailed). Data are representative of at least two independent experiments.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP nkgsaizGFFD1105AGt; zf2166Tg control Day 5 posterior intestine epithelial cell IHC
mmp9 nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
mmp13a nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
mmp14b nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 intestine RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nkgsaizGFFD1105AGt; zf2166Tg control Day 5 intestinal epithelium neoplastic, abnormal
Days 7-13 intestine mmp13a expression increased amount, abnormal
Days 7-13 intestine mmp13a expression increased amount, abnormal
Days 7-13 intestine mmp14b expression increased amount, abnormal
Days 7-13 intestine mmp9 expression increased amount, abnormal
Acknowledgments:
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