ZFIN ID: ZDB-FIG-180723-35
Enya et al., 2018 - A novel zebrafish intestinal tumor model reveals a role for cyp7a1-dependent tumor-liver crosstalk in causing adverse effects on the host.. Disease models & mechanisms   11(8) Full text @ Dis. Model. Mech.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 7-13 to Days 14-20

Fig. 5

The zebrafish intestinal tumor causes the systemic growth defect and organismal death. (A) Body length data of the sibling controls and tumor-bearing larvae at 7 and 9 dpf. The number of larvae used is 163 (7 dpf control larvae), 155 (7 dpf tumor-bearing larvae), 154 (9 dpf control larvae) and 154 (9 dpf tumor-bearing larvae). Data from three independent clutches are pooled. Data from each clutch are shown in Fig. S6. Error bars represent means±s.e.m. Statistical significance was tested using Student's t-test (unpaired, two-tailed). (B-E) Representative images of Oil Red O staining for the sibling controls (B,C) and tumor-bearing larvae (D,E) at 9 dpf. Scale bar: 500 µm. Red-stained areas represent total lipids in larvae. (F,G) Representative images of HE-stained transversal body sections for the sibling controls (F) and tumor-bearing larvae (G) at 9 dpf. Scale bar: 50 µm. (H) qPCR analysis for eif4ebp1 in the body (without the intestine or intestinal tumor) in the sibling controls and tumor-bearing larvae at 9 dpf. Scores are normalized to expression of rpl13a. The data harbors 5 biological replicates, each containing 5 larvae. Error bars represent means±s.e.m. Statistical significance was tested using Student's t-test (unpaired, two-tailed). (I) Survival rates of the sibling controls and tumor-bearing larvae from 7 to 14 dpf are shown by the Kaplan–Meier curve. Data were pooled from three independent experiments. The total number of analyzed larvae is 100 each. Statistical significance was tested using the log-rank test. Data are representative of at least two independent experiments, except I.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
eif4ebp1 nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
nkgsaizGFFD1105AGt; zf2166Tg control Days 7-13 growth decreased efficacy, abnormal
Days 7-13 whole organism decreased length, abnormal
Days 7-13 - Days 14-20 whole organism decreased life span, abnormal
Acknowledgments:
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