FIGURE
Fig. S1
Fig. S1
GFP-Dependent Activation of UAS-tdT In Vitro, Related to Figure 2 (A and B) Representative images of Gal4-GBP6VP16-GBP1 (A) and Gal4-GBP2VP16-GBP7 (B) activation of an UAS-tdT reporter in 293T cells. Cells were transfected with all indicated constructs and imaged 16 hr post-transfection. GFP, GFPmG1, DBDG and ADG constructs were delivered in separate plasmids under the CAG-promoter. Results are representative of 4 independent experiments and quantifications are tabulated in Table S2. Scale bar, 100 μm. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Cell, 154(4), Tang, J.C., Szikra, T., Kozorovitskiy, Y., Teixiera, M., Sabatini, B.L., Roska, B., and Cepko, C.L., A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation, 928-939, Copyright (2013) with permission from Elsevier. Full text @ Cell