Transgenic Construct

Tg(7xTCF-Xla.Sia:NLS-mCherry)

ID

ZDB-TGCONSTRCT-110113-2

Previous Names

Tg(7xTCF-Xla.Sia:nlsmCherry)
Tg(7xTCF-Xla.Siam:nlsmCherry)

Type

engineered_plasmid

Regulatory Regions

None

Coding Sequences

mCherry
NLS

[Em λ][Ex λ]

Fpbase:mCherry

Contains Sequences

TCF (1)

Note

This construct utilizes 7 TCF responsive elements fused to the Xenopus laevis siamois minimal promoter to drive expression of nls mCherry.

Plasmid Map

None

Genomic Features

That Utilize Tg(7xTCF-Xla.Sia:NLS-mCherry) Construct

Genomic Feature	Affected Genomic Regions
hu11828Tg
ia5Tg

Transgenics

That Utilize Tg(7xTCF-Xla.Sia:NLS-mCherry) Construct

Fish	Affected Genomic Regions	Phenotype	Gene Expression
amer1^li15/li15; ia5Tg	amer1		Fig. 4 from Große et al., 2019
apc^hu745/hu745; ia5Tg	apc
apc^sd58/sd58; ia5Tg; twu277Tg	apc	Fig. 4 from Ren et al., 2019	Fig. 4 from Ren et al., 2019
b1212Tg; ia5Tg (AB)			2 figures from Aman et al., 2018
b1212Tg; ia5Tg; w32Tg (AB)			Fig. 2 from Aman et al., 2018
bcl9^zh714/zh714; ia5Tg	bcl9	Fig. 3 from Cantù et al., 2018	Fig. 3 from Cantù et al., 2018
eda^dt1261/+; b1212Tg; ia5Tg (AB)	eda		Fig. 3 from Aman et al., 2018
eda^{dt1261/dt1261}; b1212Tg; ia5Tg (AB)	eda	Fig. 3 from Aman et al., 2018	Fig. 3 from Aman et al., 2018
f1Tg; ia5Tg
f1Tg; ia5Tg (AB)			Fig. 5 from Kalogirou et al., 2014
fb7Tg; ia5Tg			Fig. 2 from Ren et al., 2019
gars1^s266/s266; ia5Tg	gars1	Fig. S4 from Malissovas et al., 2016
gz32Tg; ia5Tg
hu10018Tg; hu11828Tg; nkuasgfp1aTg			Fig. 5 from Burkhard et al., 2018
ia5Tg		3 figures from 3 publications	11 figures from 9 publications
ia5Tg (AB)			Fig. 7 from Kalogirou et al., 2014
ia5Tg + CRISPR2-lepr		Fig. 3 from Kamstra et al., 2022	Fig. 3 from Kamstra et al., 2022
ia5Tg + MO1-fam53b + MO4-tp53			2 figures from Kizil et al., 2014
ia5Tg + MO1-ids		Fig. 6 from Costa et al., 2017	Fig. 6 from Costa et al., 2017
ia5Tg + MO1-mir7a			Fig. S5 from Adusumilli et al., 2020
ia5Tg + MO2-fam53b + MO4-tp53			2 figures from Kizil et al., 2014
ia5Tg + MO2-mir7a-1 + MO2-mir7a-2 + MO2-mir7a-3			Fig. S5 from Adusumilli et al., 2020
ia5Tg + MO3-wnt9a		Figure 3 from Paolini et al., 2021	Figure 3 from Paolini et al., 2021
ia5Tg/+			Figure 7. from Chin et al., 2020
ia5Tg/+ (AB)
ia5Tg/+ (WT)
ia5Tg/+; zn1Tg/+			Fig. 4 from Hofsteen et al., 2016
ia5Tg/+; zn1Tg/+ + CRISPR1-dab2		Fig. 4 from Hofsteen et al., 2016	Fig. 4 from Hofsteen et al., 2016
ia5Tg; jh1Tg
ia5Tg; kca3Tg; mu286Tg; s940Tg		Figure 4 from Paolini et al., 2021	Figure 4 from Paolini et al., 2021
ia5Tg; md60Tg; ubs3Tg		4 figures from Demal et al., 2019	3 figures from Demal et al., 2019
ia5Tg; md61Tg; ubs3Tg
ia5Tg; md65Tg; ubs3Tg		2 figures from Demal et al., 2019	3 figures from Demal et al., 2019
ia5Tg; md66Tg; ubs3Tg
ia5Tg; md67Tg; ubs3Tg
ia5Tg; mu107Tg		Figure 2 from Paolini et al., 2021	2 figures from Paolini et al., 2021
ia5Tg; mu201Tg; s898Tg; s940Tg		Figure 2 from Paolini et al., 2021	Figure 2 from Paolini et al., 2021
ia5Tg; nkuasgfp1aTg; pbb65Tg			Figure 1 from Paolini et al., 2021
ia5Tg; nu15Tg; y1Tg
ia5Tg; nu15Tg; y1Tg + MO1-mir7a		Fig. S8 from Adusumilli et al., 2020
ia5Tg; s704Tg		Fig. 5. from Kalasekar et al., 2019	2 figures from Kalasekar et al., 2019
ia5Tg; s750Tg; s752Tg			Fig. 1. from Kalasekar et al., 2019
ia5Tg; s843Tg			Figure 1 from Paolini et al., 2021
ia5Tg; s843Tg (AB)			5 figures from Katraki-Pavlou et al., 2021
ia5Tg; s849Tg (AB)			2 figures from Kalogirou et al., 2014
ia5Tg; s940Tg		Figure 4 from Paolini et al., 2021	4 figures from Paolini et al., 2021
ia5Tg; s940Tg + MO2-fzd9b		Figure 4 from Paolini et al., 2021	Figure 4 from Paolini et al., 2021
ia5Tg; s940Tg + MO3-wnt9a		Figure 3 from Paolini et al., 2021	Figure 3 from Paolini et al., 2021
ia5Tg; twu277Tg			2 figures from Ren et al., 2019
ia5Tg; u711Tg; zf104Tg		Fig. 1 from Guglielmi et al., 2020	Fig. 1 from Guglielmi et al., 2020
ia5Tg; w32Tg		Fig. 2 from Strand et al., 2016	Fig. 1 from Strand et al., 2016
ia5Tg; w33Tg			Fig. 5 from Ren et al., 2019
ia5Tg; y1Tg			Fig. 5 from Murayama et al., 2015
ia5Tg; y1Tg + MO3-naca		Fig. 6 from Murayama et al., 2015
ia5Tg; y7Tg
id4^bns18/+; ia5Tg	id4
id4^bns18/bns18; ia5Tg	id4		Fig. 8 from Ahuja et al., 2016
isl1a^sa29/sa29; hu10018Tg; hu11828Tg; nkuasgfp1aTg	isl1a		Fig. 5 from Burkhard et al., 2018
klf2a^sh317/sh317; klf2b^pbb42/pbb42; ia5Tg; s940Tg	klf2a klf2b	Figure 2 from Paolini et al., 2021	Figure 2 from Paolini et al., 2021
lef1^u767/u767; ia5Tg	lef1
myh6^s812/s812; ia5Tg; s849Tg	myh6	2 figures from Kalogirou et al., 2014	2 figures from Kalogirou et al., 2014
naca^{t30953/t30953}; ia5Tg	naca
ptprz1a^aa105/aa105; ia5Tg; s843Tg (AB)	ptprz1a	Fig. S4 from Katraki-Pavlou et al., 2021
ptprz1b^aa65/aa65; ia5Tg; s843Tg (AB)	ptprz1b	Fig. 3 from Katraki-Pavlou et al., 2021	Fig. S5 from Katraki-Pavlou et al., 2021
rad21a^nz171/nz171; ia5Tg/+	rad21a		Figure 7. from Chin et al., 2020
spaw^s457/s457; f1Tg; ia5Tg	spaw		Fig. 5 from Kalogirou et al., 2014
spaw^s457/s457; ia5Tg	spaw	Fig. 7 from Kalogirou et al., 2014	Fig. 7 from Kalogirou et al., 2014
spaw^s457/s457; ia5Tg; s849Tg	spaw
stag2b^nz207/nz207; ia5Tg/+	stag2b	Figure 7. from Chin et al., 2020	Figure 7. from Chin et al., 2020
tcf7l1a^m881/m881; ia5Tg	tcf7l1a
tcf7l2^zf55/zf55; ia5Tg	tcf7l2
wnt5b^ta98/ta98; ia5Tg; twu277Tg	wnt5b		Fig. 5 from Ren et al., 2019
wnt9a^sd49/sd49; ia5Tg; s940Tg	wnt9a	Figure 3 from Paolini et al., 2021	Figure 3 from Paolini et al., 2021
wnt9a^sd49/sd49; ia5Tg; s940Tg + MO3-wnt9a		Figure 3 from Paolini et al., 2021	Figure 3 from Paolini et al., 2021

Sequence Information

Other Construct Pages

None

Citations