FIGURE

Fig 9

ID

ZDB-FIG-241119-62

Publication

Coppola et al., 2024 - A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish

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Fig 9

Wnt signaling and Foxf1 require 3’reg1 to promote a surplus ACs.

A) Schematic of 3’reg1 enhancer deletion generated using CRISPR/Cas12a. Arrows indicate location of the guides. B-E) Confocal images of hearts from untreated and BIO-treated WT and 3’reg1^Δ/Δ embryos stained for Nr2f1a (green), Mhc (red), and Amhc (blue). BIO was unable to promote an increase in ACs in 3’reg1^-/- embryos. F) The number of Nr2f1a⁺/Amhc⁺ cardiomyocytes in untreated and BIO-treated WT and 3’reg1^-/- embryos. Untreated WT (n = 9); untreated 3’reg1^-/- (n = 5); treated WT (n = 11); treated 3’reg1^-/- (n = 4). G-J) Confocal images of hearts from uninjected and Foxf1 mRNA-injected WT and 3’reg1^-/- embryos stained for Nr2f1a (green), Mhc (red), and Amhc (blue). K) The number of Nr2f1a⁺/Amhc⁺ cardiomyocytes in uninjected and Foxf1 mRNA-injected WT and Nr2f1a⁺3’reg1^-/- embryos. Uninjected WT (n = 11); Uninjected 3’reg1^-/- (n = 4); Injected WT (n = 12); Injected 3’reg1^-/- (n = 4). Scale bar: 50 μm. Error bars in graphs indicate s.e.m. **** indicate P < 0.0001. ns indicates not a statistically significant difference.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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