Figure 4

A: Representative confocal images for the mouse seq132 (seq132mm enhancer chr15:52334298 + 52335281). Seq132mm enhancer showed GFP-positive cells within the endocrine pancreatic domain (dashed line) defined by the sst:mCherry reporter line. The 48-hpf embryos were stained with DAPI. Scale bars = 10 μm. Representative graph showing the total percentage of positive embryos for the sequence analyzed (13%, n = 35). *P < 0.05, by χ² test. B: Genomic landscape of the mouse Slc30a8 gene (blue), showing 4C-seq profiles (black), with view point in Slc30a8 promoter (pink asterisk) in the Min6 cell line; zoom-out (top) and zoom-in (bottom) of the Slc30a8 gene and seq132mm enhancer. The targets line represents the regions where the interaction is significant. C: sgRNAs targeting murine Slc30a8 enhancer in CRISPRa or CRISPR assay in Min6 cells. Slc30a8 expression was calculated relative to the β-actin housekeeping gene by quantitative PCR. Dot blots represent the 10–90% quantile of six biological replicates. *P < 0.05, **P < 0.01. ctrl, control.