FIGURE

Fig. 3

ID

ZDB-FIG-190910-23

Publication

O'Connor et al., 2019 - Modulation of Agrin and RhoA Pathways Ameliorates Movement Defects and Synapse Morphology in MYO9A-Depleted Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Neuromuscular junction (NMJ) morphology of myo9aa/abcrispant zebrafish at 24 hpf. Representative images of NMJs in wildtype, myo9aa/ab crispant, and NT1654 (0.15 ng) or fasudil-treated (10 µm) crispant zebrafish at 24 hpf. Acetylcholine receptors stained with αBTx (green), and motor neurons detected with an antibody against SV2 (red). White boxes demark areas enlarged in the right-hand panel, showing presence of αBTx-positive choice point clusters. Scale bar = 50 µm.

Expression Data

Antibody:	Ab-SV2
Fish:	WT WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab
Conditions:	chemical treatment by environment: fasudil hydrochloride chemical treatment by injection: peptide
Knockdown Reagents:	CRISPR1-myo9aa CRISPR1-myo9ab CRISPR2-myo9aa CRISPR2-myo9ab
Anatomical Term:	horizontal myoseptum synaptic vesicle
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab
Conditions:	chemical treatment by environment: fasudil hydrochloride chemical treatment by injection: peptide
Knockdown Reagents:	CRISPR1-myo9aa CRISPR1-myo9ab CRISPR2-myo9aa CRISPR2-myo9ab
Observed In:	horizontal myoseptum synaptic vesicle motor neuron axon
Stage:	Prim-5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells