ZFIN ID: ZDB-FIG-190910-23
O'Connor et al., 2019 - Modulation of Agrin and RhoA Pathways Ameliorates Movement Defects and Synapse Morphology in MYO9A-Depleted Zebrafish. Cells   8(8) Full text @ Cells
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Conditions:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

Neuromuscular junction (NMJ) morphology of myo9aa/abcrispant zebrafish at 24 hpf. Representative images of NMJs in wildtype, myo9aa/ab crispant, and NT1654 (0.15 ng) or fasudil-treated (10 µm) crispant zebrafish at 24 hpf. Acetylcholine receptors stained with αBTx (green), and motor neurons detected with an antibody against SV2 (red). White boxes demark areas enlarged in the right-hand panel, showing presence of αBTx-positive choice point clusters. Scale bar = 50 µm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-SV2 IHC WT standard conditions Prim-5 horizontal myoseptum synaptic vesicle
IHC WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab standard conditions Prim-5 Not Detected horizontal myoseptum synaptic vesicle
IHC WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by environment: fasudil hydrochloride Prim-5 horizontal myoseptum synaptic vesicle
IHC WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by injection: peptide Prim-5 horizontal myoseptum synaptic vesicle
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab standard conditions Prim-5 horizontal myoseptum synaptic vesicle ab-sv2 labeling absent, abnormal
Prim-5 motor neuron axon decreased length, abnormal
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by environment: fasudil hydrochloride Prim-5 horizontal myoseptum synaptic vesicle ab-sv2 labeling amount, ameliorated
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by injection: peptide Prim-5 horizontal myoseptum synaptic vesicle ab-sv2 labeling amount, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cells for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cells