|ZFIN ID: ZDB-FIG-190910-24|
NMJ morphology of myo9aa/ab crispant zebrafish at 48 hpf. Representative images of NMJs in wild-type, myo9aa/ab crispant, and NT1654 (0.15 ng) or fasudil-treated (10 µm) crispant zebrafish at 48 hpf. Acetylcholine receptors stained with αBTx (green), and motor neurons detected with an antibody against SV2 (red). White boxes demark areas enlarged in the right-hand panel. Scale bar = 50 µm.
|WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab||standard conditions||Long-pec||horizontal myoseptum neuromuscular junction of skeletal muscle fiber decreased amount, abnormal|
|Acknowledgments:||ZFIN wishes to thank the journal Cells for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cells|