ZFIN ID: ZDB-FIG-190910-22
O'Connor et al., 2019 - Modulation of Agrin and RhoA Pathways Ameliorates Movement Defects and Synapse Morphology in MYO9A-Depleted Zebrafish. Cells   8(8) Full text @ Cells
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Protruding-mouth

Fig. 1

Phenotypes of myo9aa/ab crispant zebrafish. (A) Representative images of 48 hpf zebrafish injected with sgRNAs against myo9aa and myo9ab (numbers following the gene refer to exon targeted). Injection of a combination of all four sgRNAs was performed (myo9aa/ab), along with a control against the tyrosinase gene (tyr) in which no pigment can be observed, confirming knockdown success. Scale bar = 500 µm. (B) Images showing phenotypic classification of injected zebrafish at 72 hours post fertilisation (hpf), ranging from normal to severe. Graph shows proportion of myo9aa/ab crispant fish injected with all four sgRNAs (n = 7, including 160 fish in total) as compared to wild-types (black line, n = 7, including 138 fish in total). Error bars represent mean ± S.E.M, ** p < 0.01, paired t-test.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-myo9aa standard conditions Long-pec heart edematous, abnormal
Long-pec whole organism curved, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism curved, abnormal
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab standard conditions Long-pec heart edematous, abnormal
Long-pec whole organism curved, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism curved, abnormal
WT + CRISPR1-myo9ab standard conditions Long-pec heart edematous, abnormal
Long-pec whole organism curved, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism curved, abnormal
WT + CRISPR2-myo9aa standard conditions Long-pec heart edematous, abnormal
Long-pec whole organism curved, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism curved, abnormal
WT + CRISPR2-myo9ab standard conditions Long-pec heart edematous, abnormal
Long-pec whole organism curved, abnormal
Protruding-mouth heart edematous, abnormal
Protruding-mouth whole organism curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cells for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cells