Phenotypes of myo9aa/ab crispant zebrafish. (A) Representative images of 48 hpf zebrafish injected with sgRNAs against myo9aa and myo9ab (numbers following the gene refer to exon targeted). Injection of a combination of all four sgRNAs was performed (myo9aa/ab), along with a control against the tyrosinase gene (tyr) in which no pigment can be observed, confirming knockdown success. Scale bar = 500 µm. (B) Images showing phenotypic classification of injected zebrafish at 72 hours post fertilisation (hpf), ranging from normal to severe. Graph shows proportion of myo9aa/ab crispant fish injected with all four sgRNAs (n = 7, including 160 fish in total) as compared to wild-types (black line, n = 7, including 138 fish in total). Error bars represent mean ± S.E.M, ** p < 0.01, paired t-test.