ZFIN ID: ZDB-FIG-190910-25
O'Connor et al., 2019 - Modulation of Agrin and RhoA Pathways Ameliorates Movement Defects and Synapse Morphology in MYO9A-Depleted Zebrafish. Cells   8(8) Full text @ Cells
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Knockdown Reagents:
Observed In:
Stage: Day 5

Fig. 5

NMJ morphology of myo9aa/ab crispant zebrafish at 120 hpf. (A) Representative images of NMJs in wild-type, myo9aa/ab crispant, and NT1654 (0.15 ng) or fasudil-treated (10 µm) crispant zebrafish at 120 hpf. Acetylcholine receptors stained with αBTx (green), and motor neurons detected with an antibody against SV2 (red). White boxes demark areas enlarged in the right-hand panel. Scale bar = 50 µm. (B) Colocalisation of SV2-positive signal with αBTx and (C) colocalisation of αBTx with SV2-positive signal using Mander’s correlation coefficient (0 = no colocalisation, 1 = full colocalisation). Wildtype (n = 6 fish), myo9aa/ab crispant (n = 12 fish), crispant treated with NT1654 (0.15 ng, n = 8 fish), and crispant treated with fasudil (10 µm, n = 11 fish) were subject to analysis. Error bars = mean ± S.E.M. ** p ≤ 0.01 and ns = not significant, One-way ANOVA.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab standard conditions Day 5 horizontal myoseptum neuromuscular junction of skeletal muscle fiber decreased amount, abnormal
Day 5 motor neuron axon decreased branchiness, abnormal
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by environment: fasudil hydrochloride Day 5 horizontal myoseptum neuromuscular junction of skeletal muscle fiber decreased amount, abnormal
Day 5 motor neuron axon decreased branchiness, abnormal
WT + CRISPR1-myo9aa + CRISPR1-myo9ab + CRISPR2-myo9aa + CRISPR2-myo9ab chemical treatment by injection: peptide Day 5 horizontal myoseptum neuromuscular junction of skeletal muscle fiber amount, ameliorated
Day 5 motor neuron axon branchiness, ameliorated
Acknowledgments:
ZFIN wishes to thank the journal Cells for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Cells