FIGURE

Fig. 27

ID

ZDB-FIG-190801-29

Publication

Nowak-Sliwinska et al., 2018 - Consensus guidelines for the use and interpretation of angiogenesis assays

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Fig. 27

Effects of imidazole on endothelial cell proliferation. BCECs (bovine choroidal microvascular endothelial cells) were cultured in the presence of low-glucose DMEM in the presence of 10% bovine calf serum as previously described [645]. Elution buffer was tested up to a final concentration of 5 mM imidazole, with or without 10 ng/ml VEGF165. Note that the concentration of imidazole typically used to elute His-tagged proteins is 500 mM. After 5–6 days, cell proliferation was determined by fluorescence readings at 590 nm. Asterisks denote significant differences compared to no addition (0) groups by t test (***p < 0.001, **p < 0.01)

Expression Data

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Antibody Labeling

Phenotype Data

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Acknowledgments

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