ZFIN Image: Nowak-Sliwinska et al., 2018, Fig. 27

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Figure Caption

Fig. 27

Effects of imidazole on endothelial cell proliferation. BCECs (bovine choroidal microvascular endothelial cells) were cultured in the presence of low-glucose DMEM in the presence of 10% bovine calf serum as previously described [645]. Elution buffer was tested up to a final concentration of 5 mM imidazole, with or without 10 ng/ml VEGF165. Note that the concentration of imidazole typically used to elute His-tagged proteins is 500 mM. After 5–6 days, cell proliferation was determined by fluorescence readings at 590 nm. Asterisks denote significant differences compared to no addition (0) groups by t test (***p < 0.001, **p < 0.01)

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